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Staining Jars & Racks

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Wheaton Staining Dish
These mix-and-match components offer greater flexibility in meeting your requirements.

no. 21054 to 21054-2

Description

Wheaton Staining Dishes

We offer a variety of staining dishes and Coplin jars suitable for staining in pathology, microscopy, microbiology and hematology. Staining is a contrast-enhancing procedure used to prepare samples for microscopic examination. In light microscopy, specimens are colored using various dyes, many of which are selective; that is, they stain a specific type of tissue, bacteria, or cellular component. Several stains may be used in sequence to produce a counterstained sample.

Stains are classified as acidic, basic or neutral, depending on the nature of the stain’s colored radicals. Acidic stains tend to react with cytoplasm, whereas basic stains are taken up by nucleic acids. In electron microscopy, the term “staining” may be applied to procedures wherein electron-dense materials such as salts or osmium, uranium and lead bind to and increase the electron density of various cell constituents.

For example, Aniline Blue is an acidic, water-soluble dye used to stain connective tissue. This dye is valuable for visualizing connective tissue fibers beneath epithelia within glands and muscles. Aniline Blue colors collagen fiber dark blue, mucous and cartilage matrix light blue, and nerve tissue lilac. It can be used as a counterstain with red nuclear dye. Concentrations as low as +/-1% can be used to evaluate thick sections of resin-embedded material for electron microscopy.

Dishes, covers and racks are manufactured from soda-lime glass and resist stains from Eosin or Hemotoxylin. Staining dishes are available in many configurations to accommodate up to 50 slides, with various glass and stainless steel racks.

Coplin jars can be used for pre-staining with and alcohol buffer. Slides are held vertically for easy manipulation. A Coplin jar can also serve as a preparation chamber for micro-thin-layer chromatography.

Wheaton staining dishes have been used for a variety of procedures to clean slides and coat them with a variety of materials (for example, Bain, J.R.; Hoffman, A.S. Glycophase glass revisited: Protein adsorption and cell growth on glass surfaces bearing immobilized monosaccharides, Biomaterials, in press 2002). Wheaton staining dishes have also been used for poly lysine and silane slide coatings.

Note: Wheaton staining dishes are not autoclavable or microwaveable, and are not intended for use at temperatures below 15 C or above 80 C.

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30-Slide Rack, Stainless Steel
Slotted rack is designed to hold 30 microscope slides 3″ x 1″ (75 x 25mm), 3″ x 1-1/2″ (75 x 38mm), and 3″ x 2″ (75 x 51mm).

no. 21072

Description

30-Slide Rack, Stainless Steel

Slotted rack is designed to hold 30 microscope slides 3″ x 1″ (75 x 25mm), 3″ x 1-1/2″ (75 x 38mm), and 3″ x 2″ (75 x 51mm).

The rack is made of non-tarnishable 304 stainless steel and is chemical resistant.
Handle is permanently attached, but hinged to permit closure of dish and easy insertion and removal of the microscope slides.

Rack dimensions (L x W x H): 105 x 80 x 25mm (4.13″ x 3.15″ x 1″)

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Slide Rack Adapter
Stainless steel Slide Rack Adapter kit for auto stainer slide racks.

no. 21055

Description

Slide rack adapter, ss, pkg2Slide Rack Adapter

Stainless steel Slide Rack Adapter kit for auto stainer slide racks. Allows the slide carrier rack for standard 1 x 3 slides to accommodate large slides for staining.
The Slide Rack Adapter fits into the standard slide space like a slide.
This companion adapter can be placed the appropriate distance to create a space for holding the large slide.

The Slide Rack Adapter fits most staining racks. (slide rack not included)

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16/32 Slide Rack, Glass
The Glass Rack holds 16 single slides, 31 slides arranged alternately

no. 21056

Description

16/32 Slide Rack, Glass

The Glass Rack holds 16 single slides, 31 slides arranged alternately straight across and diagonally, or 32 slides back-to-back.

Rack dimensions (L x W x H): 99 x 89 x 46mm (3.9″ x 3.5″ x 1.8″)

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20/40 Slide Rack, Glass
Holds 20 single slides or 40 back to back

no. 21057

Description

20/40 Slide Rack, Glass

Holds 20 single slides or 40 back to back of regular 3″ x 1″ (75 x 25mm) size, 3″ x 1-1/2″ (75 x 38mm) and 3″ x 2″ (75 x 51mm) sizes.

Rack dimensions (L x W x H): 119 x 87 x 48mm (4.7″ x 3.4″ x 1.9″)

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Staining Dish
Slotted rack holds 50 microscope slides

no. 21058 to 21069

Description

Staining Dish50-Slide Unit, with Cover

Slotted rack holds 50 microscope slides, sizes 3″ x 1″ (75 x 25mm) as well as 3″ x 1-1/2″ (75 x 38mm). The unit measures approximately 200 x 83 x 105mm (7.87″ x 3.27″ x 4.13″) overall.

Rack is made of non-tarnishable 304 stainless steel and is resistant to most staining solutions.
The handle is permanently attached, but hinged to permit closure of dish and easy insertion and removal of the microscope slides.

Dish and cover are made of soda lime glass.

Approximate inside dimensions: 70 x 185 x 90mm (2.75″ x 4.72″ x 3.54″).
Maximum liquid volume without rack is approximately 950 ml.

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10/20 Slide Staining Dish with Cover
For staining 3 x 1″ (75 x 25mm) microscope slides.

no. 21066

Description

10/20 Slide Staining Dish with Cover

For staining 3 x 1″ (75 x 25mm) microscope slides.

Holds 10 single slides, 19 slides arranged alternately straight across and diagonally, or 20 slides back-to-back.
Made of soda lime glass.

Approximate inside dimensions: 77 x 60 x 30mm (3″ x 2-3/8″ x 1-3/16″).
Maximum liquid volume without rack is approximately 150 ml.

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Staining Dish – 20-Slide Unit
For staining 3 x 1″ (75 x 25mm) microscope slides.

no. 21043 to 21075-6

Description

Staining Dish – 20-Slide Unit

This 20-slide unit is the standard for manual staining procedures, holds 10 single slides or 20 back to back.
Removable glass slide rack has an open bottom for rapid immersing and draining of stain, eliminating any carryover.

The rack holds 20 slides of regular size 3″ x 1″ (75 x 24mm), 3″ x 1-1/2″ (75 x 38mm) sizes.
Made of soda lime glass.

Approximate inside dimensions: 86 L x 68 W x 57mm D (3.4″ x 2.63″ x 2.25″).
Maximum liquid volume without rack is approximately 400 ml.
Rack dimensions: (L x W x H) 69 x 89 x 46mm (2.7″ x 3.5″ x 1.8″)

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Vertical Staining Jar with Cover
The wide top makes this jar especially suitable for staining slides inscribed on one end.

no. 432-1

Description

Vertical Staining Jar with Cover

The wide top makes this jar especially suitable for staining slides inscribed on one end.
Holds 8 single 3 x 1″ (75 x 25mm) slides vertically or 16 slides back-to-back.

Made of soda lime glass.
Approximate inside dimensions: 90 x 50 x 30mm (3.5″ x 2″ x 1.18″).
Holds about 50ml of liquid.

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Hellendahl Type Staining Dish
For staining or use as a TLC developing chamber

no. 21074

Description

Hellendahl Type Staining Dish

For staining or use as a TLC developing chamber.
Holds 8 individual 3″ x 1″ (75 x 25mm) slides.

If necessary, 16 slides can be accommodated back-to-back.

Made of soda lime glass.
Approximate inside dimensions: 75 x 80 x 30mm (3″ x 3.14″ x 1.18″).

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Coplin Staining Jar with Glass Cover
A popular staining jar with heavy glass walls and broad base for increased stability.

no. 21067

Description

Coplin Staining Jar with Glass Cover

A popular staining jar with heavy glass walls and broad base for increased stability.
Holds 5 single 3″ x 1″ (75 x 25mm) slides vertically or 10 slides back to back.

Requires small reagent volume.

Made of soda lime glass.
Approximate inside dimensions: 75 x 30 x 30mm (3″ x 1.18″ x 1.18″).

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Short Coplin Staining Jar with Plastic Screw Top
Holds 5 single 3″ x 1″ (75 x 25mm) slides or 10 back to back.

no. 431-3

Description

Short Coplin Staining Jar with Plastic Screw Top

Holds 5 single 3″ x 1″ (75 x 25mm) slides or 10 back to back.

Slides extend above the opening, permitting them to be manipulated without forceps.
Screw cap is linerless, white polypropylene.
The white linerless polypropylene Screw cap closure retains organic solvents and reduces evaporation.

Rectangular base.
Made of soda lime glass.
Approximate inside dimensions: 65 x 30 x 30mm (2.6″ x 1.18″ x 1.18″).
Holds about 50ml of liquid.

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Tall Coplin Staining Jar with Plastic Screw Top
For staining slides or use as a developing chamber in thin layer chromatography.

no. 428-3

Description

Tall Coplin Staining Jar with Plastic Screw Top

For staining slides or use as a developing chamber in thin layer chromatography.

Unit holds 5 single 3″ x 1″ (75 x 25mm) slides vertically or 10 slides back-to-back.
Screw cap is white linerless polypropylene which reduces solvent evaporation.

Rectangular base.
Made of soda lime glass.
Approximate inside dimensions: 70 x 30 x 30mm (2.75″ x 1.18″ x 1.18″).

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Five Slide Plastic Gripper
Holds up to five each 3″ x 1″ (25 x 75mm) slides and fits Coplin Jars and round-mouthed staining jars.

no. 21048

Description

Five Slide Plastic Gripper

Holds up to five each 3″ x 1″ (25 x 75mm) slides and fits Coplin Jars and round-mouthed staining jars.
Molded plastic withstands chemicals used in staining including special stains and metals.

Can be used in temperatures up to 80 C.

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Coplin Staining Jar with Tall Glass Cover
Chamber with extra tall lid

no. 21073

Description

Coplin Staining Jar with Tall Glass Cover

Chamber with extra tall lid accepts five single 3″ x 1″ (75 x 25mm) and 3.94″ x 1″ (100 x 25mm) slides.

Made of soda lime glass.
Meets USP Type 1 standards.

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Columbia Staining Jar for Coverslips
This staining jar can hold up to 4 cover slips

no. 21040

Description

Columbia Staining Jar for Coverslips

This staining jar can hold up to 4 cover slips measuring 17-23mm wide, and up to 30mm long.
Longer coverslips can be accommodated by removing the cap.

The jar comes with a white polypropylene screw cap with a PTFE coated polyethylene liner.

It is manufactured from Wheaton soda lime glass, which meets USP type III standards, and is resistant to most chemicals.

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Polypropylene Staining Jar
Tapered, square with flat shallow threaded screw cap.

no. 21038

Description

Polypropylene Staining Jar

Tapered, square with flat shallow threaded screw cap.
Holds either 5 single or 10 back-to-back standard 3″ x 1″ (75 x 25mm) slides in grooves.

Loosen lid before autoclaving.

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Coplin Staining Jar
Polypropylene staining jar

no. 21029

Description

Coplin Staining Jar

Polypropylene staining jar holds either 5 single or 10 back-to-back standard 3″ x 1″ (75 x 25mm) slides.

The interior is grooved to hold the slides in and upright position.
Domed and shallow thread cap provides protection to the slides.

oosen lid before autoclaving.
Sold in package of 4.

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PELCO Stain Saver Kits

no. 21092 to 2249-6

Description

PELCO Stain Saver Kits

reduce your volume of antibody and consumables to save money

  • The unique design delivers significant primary and secondary antibody cost savings (up to 90%)
  • Designed to create a capillary gap between 1″ x 3″ or 2″ x 3″ slides and any size or shape coverslip for those slides (21092)
  • There are two designs: the 21092, T-1 for single antibody staining and the 21095, T-2 designed for staining two tissues with 2 different antibodies on the same slide
  • Perfect for conventional staining protocols

This unique staining device is a breakthrough in cost-savings for immunohistochemistry. For the first time it is possible to create a capillary gap for slides or coverslips using one simple device.
There are no expensive consumables, as with other products. The design of the PELCO Stain Saver will limit the amount of precious antibody to less than 200ul for a 22 x 22mm (#2 thickness) coverslip-sized area. Total stainable area on a slide or coverslip can be up to 24 x 50mm (21092).

Either design will accommodate round or square coverslips. The tissue to be labeled can be on a coverslip or slide because both are employed to create a uniform capillary gap.
The unique adhesive properties of the mesas, in the design of the PELCO Stain Saver, provide adhesion of either Thermanox or glass coverslips to the mesa tops.

For conventional immunohistochemistry (overnight or longer incubations) the PELCO Storage Box serves as an ideal humid chamber (2239-6 holds 1 PELCO Stain Saver and the 2239-2 holds 4). Each kit contains a staining pad insert inside a black box (21092-2 Medium Black Plastic Box, styrene. T-1 and 2 kits will accommodate 3 each 1″ x 3″ or 1 each 2″ x 3″ slides. The capillary gap with a #2 coverslip is 320 m.

2249-6 and 2249-2

The Polypropylene Humid Chambers are a convenient and economical way to create a humid environment for slides, PELCO Stain Saver Kits or other slide applications. The chambers are made from sturdy, impact resistant polypropylene with a sealing lid and a polystyrene insert to place slides, PELCO Stain Saver Kits or coverslips above the water level.

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Disposable Staining Slide Tray Set
Stain, rinse and dry slides on a single working tray.

no. 21052

Description

Disposable Staining Slide Tray Set

Stain, rinse and dry slides on a single working tray.

Made of polypropylene and polyethylene blend. This rugged staining tray provides a clean, fresh working surface for up to eight slides.
Base has a deep well which holds 38ml of liquid. Top grip on lid, recessed handles on base along with two pour spouts on base, facilitate transport and waste removal.
The dark color of the base and lid make it ideal for light sensitive luminescent and UV applications.
Set includes 4 bases and 1 lid.

  • Economical alternative to metal traysSingle use avoids cross-contamination
  • Sturdy, yet light weight and compact
  • 305mm x 127mm x 29.5mm (12″ x 5″ x 1-5/32″)

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Microwave-assisted Immunostaining on Glass Slides

no. 36105 and 36107

Description

Microwave-assisted Immunostaining on Glass Slides

using the Sequenza Slide Rack and Coverplate system

The Sequenza Slide Rack, holding 10 glass slides and Coverplates, is a system designed for the immunolabeling of sections on glass slides. While originally designed for bench techniques, this system is uniquely suited for microwave-assisted applications.

The Slide Rack holds glass slides with a Coverplate. This combination forms a capillary gap between the slide and the plate.
Reagent volumes, especially antibody, are greatly reduced using this versatile system. A reagent hopper forms the uppermost part of the plastic Coverplate allowing for easy dispensing of reagent into the capillary gap.

Using a PELCO Microwave Processor with a PELCO ColdSpot, rapid immunostaining of sections on glass slides is reliable and easy.

Clean with 10% bleach solution and rinse with well water. Sequenza Slide Rack and Coverplate system is not dishwasher safe.

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Immunostain Moisture Chamber
This is a low-cost, high precision moisture chamber for ten slides.

no. 21049 to 21049-A

Description

Immunostain Moisture Chamber

This is a low-cost, high precision moisture chamber for ten slides. Every moisture chamber is divided into ten individual compartments with approximately 12.7mm (1/2″) empty space between the compartments. When the chamber lid is closed, eight barrier dividers are placed into the empty spaces between the microscope slide compartments completely isolating all compartments. This is a very desirable feature when doing immunostaining.

The microscope slides are placed on four pedestal posts and four corner posts each 11.5mm (0.460″) high, thus raising the surface of the slides approximately 12.7mm (1/2″) off the floor to keep the slides away from the water below and making the slides easily retrievable (either by hand or by forcep).

The chamber is fabricated out of heavy-duty polystyrene with an air-tight design to keep moisture in. The chamber is designed to be stackable in order to save counter and/or refrigerator space.

Dimensions: 214 x 175 x 34mm (8.43″ x 6.89″ x 1.34″)

Available in clear, black and amber.

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StainTray Slide Staining System

no. 21053

Description

StainTray Slide Staining System

for up to 10, 20 and 30 slides; available with clear or black lid

The StainTray is a versatile staining chamber for immunohistochemistry, routine and light-sensitive stains. The unique design allows water to be added in order to create a stable humidity chamber. The chamber may also be left empty and act as a waste reservoir during solution changes. There is a convenient drain plug for easy emptying of the base tray. Both the black base and black lid offer a light-safe environment for any light-sensitive stains.

The StainTray base is constructed of sturdy ABS plastic and contains 4 plastic rails, each with a silicone strip to ensure your slides adhere securely in the tray. The rails are raised to prevent interference from the water or waste in the reservoir and to make the slides easier to retrieve. The four rubber feet give greater stability to the base during staining. The units are stackable for more convenient storage. The StainTray offers multiple staining options that can be ideal for histology as well as hematology, cytology and microbiology laboratories.

  • Secure slides with convenient silicone strip
  • Black lid for light sensitive stains
  • Optional clear lid available for routine staining visibility
  • Multi-use tray as humidity chamber or waste reservoir
  • Stackable for convenient storage
  • Safe for use with cleaning agents except acetone
  • Available in 10, 20 and 30 slide capacity
  • StainTray for 30 slides dimensions: 38 x 32.5 x 4.5cm H (15″ x 13″ x 1-3/4″ H)
  • StainTray for 20 slides dimensions: 38 x 24 x 4.5cm H (15″ x 9-3/8″ x 1-3/4″ H)
  • StainTray for 10 slides dimensions: 24 x 24 x 4.5cm H (9-3/8″ x 9-3/8″ x 1-3/4″ H)

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Staining Rack, for 30 Slides, 3″
Made of welded 303 stainless steel, rugged construction.

no. 21082

Description

Staining Rack for 30 Slides, 4″

Made of welded 303 stainless steel, rugged construction.

Rack has a removable wire handle allowing the user to cover the staining dish. Holds 30 4″ long slides up to 5″ tall.
Two handles included to accommodate different slide heights.

May be used in #21054 Glass Staining Dish with Cover or #21210-66 Stainless Steel Staining Dish with Lid for the 5″ tall slides.

Dimensions: 111 x 97 x 25mm H with 101.6mm and 149mm high handles.

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Staining Rack for 30 Slides, 4″
Made of welded 303 stainless steel, rugged construction.

no. 21089

Description

Staining Rack for 30 Slides, 4″

Made of welded 303 stainless steel, rugged construction.

Rack has a removable wire handle allowing the user to cover the staining dish. Holds 30 4″ long slides up to 5″ tall.
Two handles included to accommodate different slide heights.

May be used in #21054 Glass Staining Dish with Cover or #21210-66 Stainless Steel Staining Dish with Lid for the 5″ tall slides.

Dimensions: 111 x 97 x 25mm H with 101.6mm and 149mm high handles.

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Staining Rack for 50 Slides, 3″
Rugged construction of 303 stainless steel.

no. 21088

Description

Staining Rack for 50 Slides, 3″

Rugged construction of 303 stainless steel.
Rack has a removable wire handle making it possible to cover the staining dish.

Holds 50 of 3″ long slides.

May be used in #21058 Glass Staining Dish with Cover (below) or #21210-44 Stainless Steel Staining Dish with Lid (below).

Dimensions: 178 x 89 x 25.4mm H with a 102mm high handle

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