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Citifluor Antifadent
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Citifluor antifadent mountant solutions
The use of fluorescent labels and markers is widespread and immunofluorescence, which relies on the use of antibodies tagged with labels.
no. R1320 to R1327
Description
The use of fluorescent labels and markers is widespread and immunofluorescence, which relies on the use of antibodies tagged with labels such as fluorescein, is a well-established technique. A common problem associated with the microscopical examination of these materials is that the illumination used for stimulating the fluorescence also causes degradation of the label and this causes fading of fluorescence. This is also a problem when trying to visualise materials having a low level of labelling. The photofading of labelled materials can be retarded by the use of the Citifluor mountants. They are also useful for rhodamine and DAPI.
The antifadent solutions AF1, 2 and 3 were specifically developed for use with FITC polyclonal antibodies but their use is now far more widespread. They show improved signal preservation with many other fluorochromes, including rhodamines and DAPI. AF4 contains the antifadent n-propyl gallate, which is particularly suitable for use with DAPI and Alexa dye-stained materials, as well as FITC-labelled materials
AF1
Glycerol phosphate buffered solution containing antifadent for use with labelled tissue sections and dead cells. The solution has a pH of approximately 10. Available in two sizes.
AF2
Glycerol solution containing antifadent for use with labelled tissue sections and dead cells. The solution has a pH of approximately 10. Available in two sizes.
AF3
Phosphate buffered saline solution containing antifadent for examination of whole live cells. The solution has a pH of approximately 10. Available in two sizes
AF4
Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells. An aqueous solution (75% AF4 and 25% water) has a pH of 5.
Cultures of purified cortical astrocytes with rabbit antisera against glial fibrillary acidic protein, a component of the astrocyte cytoskeleton. The rabbit antisera was visualised using fluorescein-conjugated sheep anti-rabbit 1 g antisera. Micrographs courtesy of Mark Noble, Institute of Neurology, London.
The first visual shows fluorescent using glycerol as the mountant media
The second visual shows fluorescent using Citifluor (Glycerol/PBS) as the mountant media
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Download Material Safety Data Sheet – R1320, R1320A Citifluor Glycerol Pbs Solution AF1 |
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Download Material Safety Data Sheet – R1321, R1321A Citifluor Glycerol Solution AF2 |
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Download Material Safety Data Sheet – GR1322, GR1322A Citifluor Phosphate Buffered Saline AF3 |
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Download Material Safety Data Sheet – R1327 Citifluor AF4 Mountant Solution |
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Citifluor non-fluorescent Immersion Oil
This Citifluor non-fluorescent immersion oil contains antifadent AF87.
no. R1324 and R1323A
Description
This Citifluor non-fluorescent immersion oil contains antifadent AF87.
It is designed for use at high magnifications.
It is particularly suitable for preventing the fading of dyes such as DAPI which have the unfortunate tendency to disperse in glycerol-based mounting solutions.
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Download Material Safety Data Sheet – Technical Note |
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Citifluor Solid Mountant Kit
Citifluor also provides a solid mountant.
no. R1326
Description
Citifluor also provides a solid mountant.
It is supplied as a two-component pack comprising:
- PVA solution. 25ml
- Citifluor antifadent in buffer (AF100). 5ml
The components can be mixed as required, applied to the specimen and allowed to dry to give a clear film.
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CFM solutions
The CFM solutions have been specially formulated to have a refractive index of 1.52 (similar to glass), so that effects of refraction on image quality are minimised.
no. R1330-10 to R1332-25
Description
The CFM solutions have been specially formulated to have a refractive index of 1.52 (similar to glass), so that effects of refraction on image quality are minimised. They are particularly useful for high magnification work and may also be used as immersion oils. They are valuable for laser scanning microscopy where three dimensional imaging of specimens is carried out. CFM solutions should only be used with fixed (cross-linked) samples.
CFM 1.
Glycerol phosphate buffered saline solution. Available in two sizes.
CFM 1 plus antifadent.
Glycerol phosphate buffered saline solution containing antifadent. Available in two sizes.
CFM 2.
Glycerol tris buffered solution. Available in two sizes.
CFM 3.
Glycerol phosphate based solution containing antifadent. Available in two sizes.
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UV Mount Media (UVM)
Methacrylate-based solution containing an antibleaching agent
no. R1333-2
Description
UV mountants harden to give a clear film when exposed to light. They fix the specimen upon hardening, making microscopic examination of specimens that move around or change shape on the slide much easier.
The solutions are water miscible prior to curing and so can be used with non-dried specimens.
UVM 2
Methacrylate-based solution containing an antibleaching agent
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Download Material Safety Data Sheet |
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Antifadent (AF) solutions
Phosphate buffered saline solution containing antifadent, specially prepared for use with UVM 3 solution where fluorochrome fading is a problem.
no. R1328-1 to R1328-3
Description
AF100
Phosphate buffered saline solution containing antifadent, specially prepared for use with UVM 3 solution where fluorochrome fading is a problem. Only available in conjunction with an aqueous solution of poly(vinyl alcohol) or with UVM 1 and UVM 3.
AF200
Glycerol solution containing antifadent, specially prepared for use with UVM 2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dyes.
AF300
Glycerol solution containing antifadent, specially prepared for use with UVM 2 solution where fluorescein is the staining material. The solutions can also be used with other fluorochromes such as rhodamines etc
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Download Material Safety Data Sheet – R1328-1 Citifluor AF100 Antifadent Solution |
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Download Material Safety Data Sheet – R1328-2 Citifluor AF200 Antifadent Solution |
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Download Material Safety Data Sheet – R1328-3 Citifluor AF300 Antifadent Solution |
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