Products
PELCO BioWave Pro+
The future of microwave tissue processing is here…
The new design of the PELCO BioWave® Pro+ introduces features that improve efficiency and streamline functionality.
It is built on trusted technology and adds enhancements for efficient laboratory processing.
This new tissue processor further enhances processing of tissue for diagnostic and research TEM, immunofluorescence and confocal specimen processing.
The PELCO BioWave® Pro+ meets the current needs of the modern laboratory with up-to-date technology.
Clickable text navigation allows for simple and clear screen interaction.
Live run-time graphs provide an easily accessible view of the current protocol in real-time.
The simplified protocol selection screen allows one-touch access to loaded and custom protocols.
User-Friendly Run Screens
Intuitive functions in a simple, consistent format offer all setting controls within one screen.
Live Run-Time Graph
Quick and convenient view of the current protocol in real-time provides an active view of run progress.
Simplified Protocol Selection
Icon-driven interface allows users one-touch access to pre-loaded and customized protocols.
The PELCO BioWave® Pro+ fits nicely into a core facility or multi-user environment by providing options to users for data manipulation.
Each user is able to create a folder for their protocols and recorded data on the PELCO BioWave® Pro+ directly or may choose to keep their data and protocols on a USB key.
The new PELCO BioWave® Pro+ Report/Protocol Manager app facilitates data transfer and review, as well as custom protocol creation and upload.
PELCO BioWave® Pro+ RPM App
The Report/Protocol Manager (RPM) app is designed to meet diagnostic laboratory regulations on reviewing recorded data for each protocol.
Recorded data can be downloaded to the USB key and then transferred into the RPM app on a laboratory computer.
The RPM app also provides an improved design for protocol creation and review.
The completed protocol can be saved to the USB key and then imported to the PELCO BioWave® Pro+ directly from the touchscreen.
Built on Trusted Technology
The PELCO BioWave® Pro+ was designed using over 25 years of proven experience in microwave tissue processing. The incredible efficiency of the PELCO BioWave® Pro+ has improved electron microscopy processing to be 95% faster than traditional bench processing.
Improvements and enhancements continue to highlight the advantages of the PELCO BioWave® Pro+ for many facets of laboratory use.
Unmatched Control
The PELCO BioWave® Pro+ offers a unique and unparalleled level of control over microwave processes for reliable and reproducible results. By being designed to properly use microwave energy, the PELCO BioWave® Pro+ provides the tools for control of the environment at the specimen level and the internal chamber.
Variable Wattage and PELCO ColdSpot® Technology
Variable wattage provides an essential avenue of control for the PELCO BioWave® Pro+. As compared to “pulsed power” microwaves, variable wattage allows the user to limit the exposure of any specimen to the minimum energy necessary to process the specimen. The patented ColdSpot® technology alleviates inconsistent wattage delivery in the microwave cavity, guarding the specimens from excessive microwave energy.
Versatility and Reproducibility
The versatility of the PELCO BioWave® Pro+ is demonstrated through the diverse Application Kits available for various laboratory procedures. A diverse and well-thought out set of accessories offers a wide range of applications with just one instrument. Accessories designed specifically for each microwave application provide for easy reproducibility.
Internal Load Cooler & Vacuum
The PELCO BioWave® Pro+ is designed as a complete system. For control of the internal environment, the ColdSpot® technology is coupled with an internal Load Cooler and requires no outside instrumentation.
By providing a built-in vacuum pump, no external connections or equipment are necessary.
This kit provides a full range of accessories designed for efficient EM processing in the microwave, enabling complete process from fixation to resin.
The versatility of this kit equips the user for multipletypes of staining protocols.
All accessories are compatible with immunohistochemistry and immunofluorescencestains and reagents.
All the accessories needed for complete paraffin processing are included in this kit.
Vacuum-assisted infiltration, along with
microwave energy, enhances any standard protocol.
The unique components of this
kit provide a true advantage to
the decalcification process.
Microwave technology provides notable savings of time and reagent, offsetting decalcification’s most significant limitations.
| Dimensions | 553mm x 514mm x 546mm (21.75″ W x 20.25″ D x 21.5″ H) |
| Weight | 37kg (83lb) |
| Microwave Power Range | Continuous power from 100 – 750 watts |
| Microwave Frequency | 2.45 Ghz |
| User Interface | 7″ Touchscreen |
| Temperature Probe | ±1°C |
| Magnetic Stirrer | Integrated, 0 – 3000rpm speed |
| Exhaust | 110 cfm capacity |
| Vacuum System | 20″ Hg, 3 modes |
| Certification | ETL/CE |
| Power | 36700: 120V, 15amp, 60Hz dedicated circuit 36700-230: 230V, 10amp, 50Hz dedicated circuit |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36700-230 | PELCO BioWave Pro+ Microwave Processing System, 230VAC | Each | p.o.r. |
Equipment, Accessories & Consumables
designed as a temperature controlled surface
will enhance most tissue processing steps
The PELCO ColdSpot Pro is designed for microwave processing techniques that benefit from an efficient heat transfer between the PELCO ColdSpot surface and the sample.
This product design provides excellent temperature control for electron microscopy tissue processing, immunolabeling and in situ hybridization protocols.
The glass surface dissipates heat quickly and efficiently. This design creates a flat surface across the entire glass top enabling a wide range of usage with consistency.
The temperature of the water inside this PELCO ColdSpot should not exceed 60 C (140 F) because the thermal properties of the plastic and the adhesive will be compromised at higher temperatures.
All PELCO Microwave processors can be set to maintain the temperature below this critical point. Designed for use with 3536 PELCO Vacuum Chamber.
Glass plate area dimensions: 223 W x 291mm D (8.78 W x 11.34″ D)
Overall dimensions: 267 W x 381 D x 108mm H (10.5 W x 15 D x 4.25″ H)
Technical Note
 | Download Technical Note |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36116-10 | PELCO ColdSpot Pro | Each | € 1.475,10 |
designed for higher solution temperatures
The PELCO ColdSpot Plus is designed for microwave processing techniques that benefit from retained heat and higher solution temperatures.
The unique construction of this PELCO ColdSpot is designed for the temperature requirements encountered during paraffin processing applications.
The all-welded construction combined with the solvent resistant and thermal properties of the plastic make it the ideal choice when processing solution temperatures of 50 C (122 F) or higher are required.
This PELCO ColdSpot Plus design provides control of the microwave environment while meeting the demands presented during high temperature processing techniques.
Top work area dimensions: 267 W x 286mm D (10.5 W x 11.25″ D)
Overall dimensions: 267 W x 356 D x 105mm H (10.5 W x 14 D x 4.125″ H)
Technical Note
| Download Technical Note |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36116-20 | PELCO ColdSpot Plus | Each | € 999,60 |
PELCO SteadyTemp Pro
Solid State Chiller with Digital Control
Fully integrates with the PELCO BioWave Pro+ Microwave System to provide temperature control at the sample level.
The PELCO Steady Temp Pro is specially designed to work in tandem with the PELCO BioWave Pro+ microwave system.
This chiller provides temperature control at the sample level within the microwave chamber.
The technology of the PELCO ColdSpotPro combined with the PELCO SteadyTemp Pro Solid State Chiller offers the unique ability to provide continuous temperature control from 4 C to 80 C.
The compact design, fully integrated controls linked to the PELCO BioWave Pro+ system, and reliable Freon-free technology make the PELCO SteadyTemp Pro an essential accessory for any laboratory microwave processing application.
| Exterior Dimensions: | 234 x 280 x 324mm (12.75″ x 11″ x 12.75″) |
| Weight: | 61.6 kg (28 lb) |
| Electrical: | Universal Voltage 115-230 VAC, 50/60 Hz, 7/5 A |
| Noise Level: | 60 dba. |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 50062 | PELCO SteadyTemp™ Pro Digital Thermoelectric Recirculator | Each | € 7.361,30 |
Processing
PELCO HistoWave Cassette SystemCan be used as a stand alone system or in the PELCO TissueVac accessory. | |||
 | |||
| Cassette Rack shown assembled and loaded. Note the two over-sized (white) cassettes being accommodated in the center of the rack. | |||
| The PELCO HistoWave Cassette System consists of a Cassette Tank and Cassette Rack. The design provides for ease of loading, unloading and fluid exchange during processing. The system will hold 26 standard-sized plastic cassettes or 22 standard and 2 over-sized cassettes. There is a hole in the handle for the temperature probe. The amount of reagent needed to cover 1 or 26 cassettes is approximately 500ml. It is easiest to add solutions when the system is assembled. The system is made of microwave-compatible and solvent-resistant plastics. Use the optionally available Paraffin Heating Tile, Prod. No. 36150-1, under the Cassette Tank when more heat generation is required in the Microwave Tissue Processor for paraffin embedding. Note: The PELCO HistoWave Cassette System may also be used in the PELCO TissueVac Vacuum Chamber, Prod. No’s 36161 and 36160, which is an accessory for the PELCO BioWave Pro and previous PELCO Microwave Processors.
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 |  | |  |
| Cassette Tank | PELCO HistoWave Cassette System loaded and assembled. | Cassette Holder with cassettes may be rested on tank to facilitate draining. | Optional Paraffin Heating Tile |
| Dimensions of Cassette Holder (with handle): Size of Standard Histology Cassette: Size of Over-Sized Histology Cassette: | |||
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36150 | PELCO HistoWave Cassette System | Each | € 569,70 | ||
| 36151 | PELCO HistoWave Cassette Tank only | Each | € 366,50 | ||
| 36151-1 | PELCO HistoWave Cassette Rack only | Each | € 400,30 | ||
| 36150-1 | PELCO HistoWave Paraffin Heating Tile | Each | € 67,90 |
PELCO Prep-Eze Microwave-use Specimen Holders
The PELCO Prep-Eze Holder accomodates processing 6-12 specimen batches.
The unique design provides simpler handling and reduction in the volume of chemicals needed.
The PELCO Prep-Eze fits with the 36135 Polypropylene Petri Dishes.
The outside dimensions of the holders are: 50.8mm diameter x 19mm High 44.5mm High with handle)
No. 36157-1 has 6 wells, 12.7mm diameter x 17mm deep.
No. 36158-1 has 12 wells, 9.5mm diameter x 17mm deep.
The mesh openings are 420 m.
The small hole can be used to hold the temperature probe for specimen level temperature control.
 |  |
| 36157-3 | 36158-3 |
Numbered mats, available for both sizes, are plastic laminated to resist chemicals and may be written on with a dry erase marker.
With the holder placed on the mat, easy identification of specific wells is possible.
The kits includes one specimen holder, one ID mat and 20 polypropylene petri dishes (58 x 15mm, Prod. No. 36135) suitable for use in the microwave.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36157 | PELCO Prep-Eze™ 6-well Kit | Each | € 151,00 | ||
| 36157-1 | PELCO Prep-Eze™ 6-well Holder | Each | € 119,50 | ||
| 36157-3 | PELCO Prep-Eze™ 6-well I.D. Mat | 3 | € 34,70 | ||
| 36158 | PELCO Prep-Eze™ 12-well Kit | Each | € 180,80 | ||
| 36158-1 | PELCO Prep-Eze™ 12-well Holder | Each | € 166,40 | ||
| 36158-3 | PELCO Prep-Eze™ 12-well I.D. Mat | 3 | € 34,70 |
PELCO Microwave Microcentrifuge Tube Holder
Made from PTFE, designed to hold 18 conventional polyethylene microcentrifuge tubes.
Firmly holds tubes in water bath or on PELCO ColdSpot Pro during processing.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36134 | PELCO Microwave Microcentrifuge Tubes PTFE Holder | Each | € 75,80 |
PELCO Microwave Capsule Holder with Lid
The improved PELCO Microwave Capsule Holder has three design features that set it apart from the previous design.
- “Stops” on the legs to keep the capsule base level during loading
- A hole with a stop in the base to allow the 00 embedding capsule to be set in place easily
- The new capture top that has a recess to keep the embedding capsule lid in place throughout the polymerization process in the microwave
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36131-2 | PELCO Microwave Capsule Holder with Lid | Each | € 283,30 |
Polypropylene Petri Dishes
Polypropylene Petri Dishes are microwave transparent and sturdy.
These dishes can be coupled with the PELCO Prep-Eze holders for microwave processing.
Bottom inside dimensions: 50mm dia. x 12mm deep
Lid inside dimensions: 56mm dia. x 10mm deep
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36135 | Polypropylene Petri Dishes, 50mm ID x 12mm, pk/100 | Each | € 48,10 | ||
| 36135-5 | Polypropylene Petri Dishes, 50mm ID x 12mm, pk/500 | Each | € 213,50 |
| Microwave Resin Polymerization System Designed for resin polymerization using the 36131 or 36131-2 Capsule Holders in the PELCO BioWave Microwave Tissue Processor During microwave-assisted resin polymerization the microwave polymerization system is placed on the Microwave Polymerization Stand on the PELCO ColdSpot Pro. The insulator pad is placed on the surface of the water and the lid placed on the tray. This new system controls the rate of heating and the temperature maximum (100 C) without the loss of water during the polymerization process. Microwave wattage will control the heating rate which ensures a quality polymerization each run. | |||
 |  | ||
| Microwave Polymerization System shown with the Polymerization Stand (36133-20) and PELCO Microwave Capsule Holder (36131-2) on the PELCO ColdSpot Pro (36116-10) | Microwave Polymerization Stand (36133-20) | ||
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36133 | Microwave Polymerization System | Each | € 48,10 | ||
| 36133-10 | Replacement Insulator Pads, 1/2″ thick | 3 | € 20,90 |
Corning Costar Tissue Culture Plates
Tissue Culture-treated Plates are designed for a wide range of applications including general cell growth experiments, cloning studies, virus isolation and in vitro testing.
Plate bottoms have a uniform thickness, allowing for distortion-free observation. Alphanumeric coordinates are placed on the same focal plane as cell growth, for convenient referencing of cell position. Gripping edges make handling easier.
All plates have non-reversible covers with condensation rings to minimize evaporation and the risk of contamination.
Plates are made of optically clear, medical-grade non-pyrogenic polystyrene, TC-treated, sterile and are used for processing of tissue on the bench or in the microwave. The corresponding Wellplate Inserts are No. 36168 for the 6 cavity, No. 36170 for the 12 cavity, and No. 36172 for the 24 cavity.
Ordering Information
| No. of Wells | Well Dia. | Well Growth Area | Total Well Volume | Working Volume | |
|---|---|---|---|---|---|
| 36168-6 | 6 | 35mm | 9.5cm2 | 15ml | 2-3ml |
| 36170-12 | 12 | 22.6mm | 3.8cm2 | 7ml | 1ml |
| 36172-24 | 24 | 16mm | 1.9cm2 | 3.4ml | 0.5ml |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36168-6 | Tissue Culture Plate, 6 wells | 10 | € 79,20 | ||
| 36170-12 | Tissue Culture Plate, 12 wells | 10 | € 87,00 | ||
| 36172-24 | Tissue Culture Plate, 24 wells | 10 | € 96,30 |
Amplate Roller
Manufactured with a comfort grip to assist in creating a perfect seal when used with SecureSeal Sealing Film.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36167 | Amplate roller | Each | € 57,60 |
8-Section Staining Nets
Dimensions: 89 x 38mm.
Available with netting of 32 mesh or 64 mesh
Rod in center of net can be grasped to lift net from Staining Dish.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36154-32 | 8-Section Staining Net, 32 mesh | Each | € 148,60 | ||
| 36154-64 | 8-Section Staining Net, 64 mesh | Each | € 148,60 |
Pyrex Staining Dish
100mm diameter x 50mm H, for use with above Staining Nets.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36754-60 | Pyrex Staining Dish | Each | € 80,30 |
Staining
PELCO Prep-Eze Wellplate Inserts
The rectangular polypropylene Wellplate Inserts have 6, 12, or 24 wells and will fit into the Corning Costar Tissue Culture Plates of 6/12/24 Wellplate design. A 420 m opening, polypropylene mesh is at the bottom of each of the insert wells.
The Wellplate Inserts may only be chemically sterilized.
The outside dimensions of the Wellplate Inserts are: 81.3mm W x 123.5mm L x 24mm H
Well dim. of 6-wellplate inserts: 24.1mm dia x 22.3mm D
Well dim. of 12-wellplate inserts: 14.2mm dia x 22.3mm D
Well dim. of 24-wellplate inserts: 11.2mm dia x 22.3mm D
Depth of well from top to bottom on all Wellplate Inserts: 17.68mm
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36168 | PELCO Prep-Eze™ 6-wellplate Insert | Each | € 145,80 | ||
| 36170 | PELCO Prep-Eze™ 12-wellplate Insert | Each | € 140,30 | ||
| 36172 | PELCO Prep-Eze™ 24-wellplate Insert | Each | € 316,80 | ||
| 36168-8 | 6-well Insert (or 24.1mm dia.) Replacement Mesh, 420 m | 30 | € 46,10 | ||
| 36170-14 | 12-well Insert (or 14.2mm dia.) Replacement Mesh, 420 m | Each | € 46,10 | ||
| 36170-24 | 24-well Insert (or 11.2mm dia.) Replacement Mesh, 420 m | 48 | € 46,10 |
Individual Wells
Great for processing individual specimens or keeping specimens separate from others.
Same wells as used in the Wellplate Inserts above but on an individual basis.
Made entirely from polypropylene.
Available in three sizes, all with 420 m openings in the screen.
Dimensions of the Individual Wells:
| Outside Diameter | Inside Diameter | Deep | Opening | |
|---|---|---|---|---|
| Individual Well 6 #36169 | 31.7mm | 24.1mm | 22.3mm | 420 m |
| Individual Well 12 #36171 | 19.25mm | 14mm | 22.3mm | 420 m |
| Individual Well 24 #36173 | 14.55mm | 11.2mm | 22.3mm | 420 m |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36169 | Individual Well, 24.1mm I.D. x 22.3mm Deep, 420 m opening screen, all polypropylene | Each | € 38,20 | ||
| 36171 | Individual Well, 14.2mm I.D. x 22.3mm Deep, 420 m opening screen, all polypropylene | Each | € 23,50 | ||
| 36173 | Individual Well, 11.2mm I.D. x 22.3mm Deep, 420 m opening screen, all polypropylene | Each | € 13,80 |
Microwave-assisted Immunostaining on Glass Slides
using the Sequenza Slide Rack and Coverplate system
The Sequenza Slide Rack, holding 10 glass slides and Coverplates, is a system designed for the immunolabeling of sections on glass slides. While originally designed for bench techniques, this system is uniquely suited for microwave-assisted applications.
The Slide Rack holds glass slides with a Coverplate. This combination forms a capillary gap between the slide and the plate.
Reagent volumes, especially antibody, are greatly reduced using this versatile system. A reagent hopper forms the uppermost part of the plastic Coverplate allowing for easy dispensing of reagent into the capillary gap.
Using a PELCO Microwave Processor with a PELCO ColdSpot, rapid immunostaining of sections on glass slides is reliable and easy.
Clean with 10% bleach solution and rinse with well water. Sequenza Slide Rack and Coverplate system is not dishwasher safe.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36105 | Sequenza Slide Rack | Each | € 1.367,20 | ||
| 36107 | Coverplate Assemblies | 25 | € 297,60 |
Vacuum
PELCO TissueVac®
Histology Vacuum Accessory
Vacuum Chamber and Embedding – Processing Cassette Holder – for Microwave Processing
ELCO TissueVac® Accessory shown with Cassette Holder
in Vacuum Chamber / Processing Container with Temperature Probe inserted
The PELCO TissueVac® Histology Vacuum Accessory is designed for the PELCO BioWave® Pro+ Microwave Processor and can be used in conjunction with the PELCO ColdSpot®.
It can be used with any of the three vacuum settings on the PELCO BioWave® Pro (vacuum cycle, vacuum on W/MW, vacuum on) or without the vacuum function.
The container and lid are made from microwave-transparent polypropylene. The integrated temperature probe allows the use of temperature restriction conrol with vacuum.
The cassette holder will hold up to 58 standard size plastic cassettes, all in the same orientation and plane. This assures that every tissue sample will see the same microwave conditions.
The paraffin heating tile is used as a heat receptor during paraffin infiltration.
The PELCO TissueVac® System is comprised of the following components:
- Processing Container (36160-1)
- Lid Assembly (36160-2)
- Cassette Holder (PTFE) for 58 cassettes (36160-3)
- Paraffin Heating Tile (36160-4)
- PTFE coated Temperature Probe (36160-5)
- L-gasket (36160-6); Nylon Locking Strap (36160-7)
- Operation Manual.
 |  |  | Cassettes loaded into Round Cassette Holder 36160-3 | Top piece put in place | Paraffin Heating Tile |
Vacuum Chamber outside dimensions:
169.9mm H (with handle) x 277.8mm W (with handles)
(6.69″ H x 10.09″ W)
Vacuum Chamber inside dimensions (with paraffin heating tile and lid in place):
92.2mm H x 177.3mm dia. (3.63″ H x 6.98 dia.)
Cassette Holder dimensions (with handle):
7.94cm H (with handle) x 17.5cm dia. (3.125″ H x 6.875″ dia.)
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 36160 | PELCO TissueVac System | Each | € 1.468,60 | ||
| 36160-1 | Processing Container | Each | € 544,50 | ||
| 36160-2 | Lid Assembly for 36160 | Each | € 568,80 | ||
| 36160-4 | Paraffin Heating Tile for 36160 | Each | € 149,50 | ||
| 36160-5 | PTFE coated Temperature Probe | Each | € 337,10 | ||
| 36160-6 | L-gasket for 36160 | Each | € 74,10 | ||
| 36160-7 | Nylon Locking Strap | Each | € 4,80 |
PELCO EM Pro Vacuum Chamber
Features
- Made of chemically resistant, microwave-transparent polypropylene and designed for vacuum down to 20″ of Hg (1 torr/1mm Hg) and temperatures up to 70 C.
- Designed either to run a continuous vacuum during microwave processing or a vacuum cycle.
- Improves fixative penetration and ultrastructural preservation during microwave-assisted chemical fixation.
- Improves resin infiltration in the microwave and greatly reduces the turnaround times when compared to established microwave protocols.
Can be used in or outside of the PELCO BioWave Pro Microwave Tissue Processor.
Dimensions
| Inside Chamber | 178mm diameter x 84mm height |
| Overall Dimensions | 203mm diameter x 111mm height |
 |  |
| Top Plate with Temperature Probe Port (optional). Note that the Temperature Probe with cable connection for the PELCO BioWave Pro Microwave Tissue Processor is inserted (also optional) See below for all accessories. | PELCO EM Pro Vacuum Chamber shown with optional Top Plate with temperature probe port and optional Temperature Probe in place. |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 3536 | PELCO EM Pro Microwave Vacuum Chamber, with Top Plate, Base Unit with L-gasket Vacuum Seal and 1/4″ Barb Fitting for vacuum hose attachment | Each | € 1.246,50 | ||
| 3435-1 | Replacement Vacuum Chamber L-gaskets | 2 | € 188,80 | ||
| 3536-10 | Top Plate with Temperature Probe Port (optional accessory) | Each | € 469,40 |