Products
UltrAuFoil® Holey Gold Films
- Apertures
- Apertures 1,85 x 0,1mm
- Apertures 2,0 x 0,1mm
- Apertures 2,0 x 0,25mm
- Apertures 2,0 x 0,6mm
- Apertures 3,0 x 0,1mm
- Apertures 3,0 x 0,25mm
- Apertures 3,04 x 0,25mm
- Apertures 4,0 x 0,2mm
- Apertures 4,0 x 0,6mm
- Apertures 6.35 x 0.02mm
- Apertures 6,35 x 0,125mm
- Apertures 10,0 x 0,1mm
- Apertures 10,0 x 0,6mm
- Apertures 12,0 x 0,1mm
- Apertures 19,99 x 0,25mm
- Gilded strip apertures
- Multihole Strip Apertures
- Spray Apertures
- Thin Foil Apertures
- Tantalum Wehnelt
- Top Hat Apertures
- Aperture Cleaning
- Brushes
- Calibration
- GSR & Particle Analysis
- Light Microscopy
- SEM Calibration
- (CD) Calibration Test
- Back Scattered Electron Test
- Boron substrates
- Chessy test specimen
- Critical Dimension Magnification Standards
- Demonstration Specimens
- Glass size standards
- Gold on Carbon
- Line/Grid Standards
- Low & Medium Magnification
- MRS-3
- MRS-4
- MRS-6
- Polystyrene Spheres
- Silicon test specimen
- Tin on Carbon
- TEM Calibration
- Chemicals
- Cleaning
- Cryo Products
- Desiccators Cabinets
- Diamond Knives
- Dissection
- Embedding
- FIB Supplies
- Filaments
- Forensics
- General Lab Supplies
- Applicator, Sticks and Swabs
- Beakers, Cylinders and Containers
- Cell Strainer System
- Dishes and Boats
- Glass Bottom Dishes
- Laboratory Glassware
- Laboratory Tongs
- Microscopy Illuminators
- Microwave Specimen Holders
- Parafilm M®, Melinex Film
- PELCO Rotators
- Petri Dishes
- Pocket Scales
- Spatulas & Spoons
- Storage Jars, Containers & Bottles
- Thermometers
- Timers
- Ultraviolet Lamps
- Vials, Tubes and Cups
- Wire Mesh
- Gloves
- Grids
- Histology
- Immersion Oils
- Laboratory Tape
- Microscope Slides
- Pipettes and Pipettors
- PTFE Laboratory Products
- Razor- Microtome Blades
- Safety Products
- Slide Boxes, Trays & Mailers
- Slide Labels
- Spatulas and Spoons
- Staining
- Weighing Dishes
- Black Plastic Products
- Blades
- Brain and Tissue Matrices
- Citifluor Solutions
- Diamond Microtome Knives
- Embedding Cassettes
- Glass Bottom Dishes
- Instruments
- Microscope Slides
- PAP Liquid Blocker Pens
- PTFE Products
- Slide Boxes
- Small Animal Research
- Specimen Holders
- Staining Jars & Racks
- Tools for Neuroscience
- Carbon tabs, tapes & sheets
- Mount Storage Boxes
- Planchets
- Replication Materials
- Scintillators
- Specimen Mount Grippers
- Specimen Preparation Stations
- Starter Kits
- Stubs, Mounts and Holders
- M4 Threaded, Cylinder, and Other Stubs
- Bulk Specimen Holders, M4
- Carbon Planchet Holders, M4
- Carbon, Brass, and Copper Mounts
- Cross Section Holders
- Cylinder Specimen Mounts
- E-Beam Lithography Mount, M4
- EBSD Pre-tilt Holders & Mounts, M4
- Filter Holders, M4
- Flat Sample Clip Holders
- Hitachi T-base Specimen Holders
- Metallographic Mount Holders
- Modular Stage Adapters, M4
- Mount Adapters, M4
- Multipurpose Specimen Holder
- Pin Stub Holders, M4
- Q Pin Mounts and Holders
- Rectangular Mounts
- SEMClamp Holders, M4
- SEMClip Cylinder Mounts
- SEMClip Geological
- STEM Imaging Holder, M4
- TEM Grid Holder, M4
- Tilt Specimen Holders
- Vise Type Holders
- Wafer & Large Sample Holders
- Pin Stubs Specimen Mounts and Holders
- Adapter Buttons
- Angled Pin Stub Holders
- Bulk Specimen Holders with Pin
- Carbon, Brass, and Copper, SEM Mounts
- E-Beam Lithography Mount
- EBSD Pre-tilt Holders & Mounts
- FIB Grid Holders
- Filter Holders, Pin
- Jeol Specimen Holders
- Low Profile FIB Mounts
- Low Profile SEMClip
- Metallographic Mount Holders
- Modular Stage Adapters
- Mount Adapters
- Multi Purpose / Bulk Holders
- Multi-Pin Stub Holders
- Pin Stub Specimen mounts
- Planchet Holders
- Q Pin Stubs and Holders
- Sample Holder Sets
- SEMClamp Holders
- SEMClip Pin Mounts
- Slotted Pin Stubs
- STEM Imaging Holder
- TEM Grid Holders
- Thin Section Holders
- Variable Tilt Holders
- Vise Clamp Holders
- Wafer Holders with Pin
- M4 Threaded, Cylinder, and Other Stubs
- Cabinets for Tissue Cassettes
- Cardboard, Tin Boxes & Cans
- Gel-Pak® Substrate Carriers
- Grid Storage Boxes
- Compact Modular Cabinet
- Plastic Jars, Containers & Bottles
- Membrane Boxes
- Plastic Storage Boxes
- Slide Boxes, Trays and Mailers
- Mount Storage Boxes
- Wafer Carrier Trays
- PELCO X-TREME Cases
- Zipper, Barrier Bags
- Accessories
- Aesculap® – Forceps
- Aquarius Tweezers
- Cantilever Tweezers
- Carbon Fibre tweezers
- Dumont™ Tweezers
- General Purpose Forceps
- Industrial, Economy Tweezers
- Mount Grippers
- PELCO Tweezers
- Plastic Tipped ESD Tweezers
- Plastic Tweezers, Flat & Sharp
- Plastic, Wafer Tip Styles
- Surgical Tissue Forceps
- Wafer Tweezers
- Wafer, Component, Cover Glass
- Wire Cutting Tweezers
- Zirconia Ceramic Tweezers
For electron cryo-microscopy

Modified from “From Christopher J. Russo, Lori A. Passmore, SCIENCE 346: 1377, 2014 (DOI: 10.1126/science.125953).
Reprinted with permission from AAAS.”
One of the major problems when performing electron cryo-microscopy (cryo-EM) is specimen movement from beam irradiation during imaging, which results in blurred, low resolution images.
One cause of excessive specimen drift is the use of carbon foils on metal (Cu) grids, which can move up to 400Å parallel to the beam. The UltrAuFoil® grids were specifically developed for cryo-EM, to reduce the movement of cryo-specimens during imaging.
The UltrAufoil® grids are a 200 or 300 mesh gold grid with a 400-500Å thick polycrystalline gold foil, which have a regular square array of micrometer-sized circular holes.
These grids are similar to Quantifoil® Grids except that the UltrAuFoil® grids use gold foil instead of a carbon foil and are less fragile. Similar to the carbon supports, the UltrAuFoil® grids can be made hydrophilic using low-energy plasma.
The UltrAuFoil® grids can improve your image quality by reducing radiation-induced motion without the use of sophisticated drift correction or hardware.
Two different mesh sizes are available with three different hole patterns:
- 200 mesh grids, R 2/2
- 300 mesh grids, R 0.6/1 or R 1.2/1.3
The UltrAuFoil® grids were designed by Drs. Lori A. Passmore and Christopher J. Russo at the Medical Research Council’s Laboratory of Molecular Biology and produced by Quantifoil® Micro Tools.*
Advantages of UltrAuFoil®:
- The gold foil on a gold grid prevents the foil from changing shape when the sample is cooled during preparation
- Chemically inert and biocompatible
- Less fragile than carbon foils, highly conductive, & non-oxidizing
- The grids have no expiration date, but are recommended to be used within two years
- The foil is ready to use when delivered, but can be made more hydrophilic using the PELCO easiGlow™ Glow Discharge Cleaning System
- A continuous film of carbon can be added using a standard float transfer method
Some tips on using UltrAuFoil®:
- When setting up the beam for data collection, use a circularly symmetric beam geometry, center it over a hole with a small region of the support around the edge, and take an image of the center of the hole.
- The easiest way to focus on the grids is to minimize the image shift by using the beam tilt wobble OR remove the objective aperture and find diffracted beams at the edge of a hole, and then minimize the shift between the crystals of gold and the diffracted beams.
- For correcting the astigmatism, it is best to use a calibrated standard before imaging the sample.
- Sample collection can be automated using the beam tilt to focus.
*References:
Russo, C. J., & Passmore, L. A. (2014). Ultrastable gold substrates for electron cryomicroscopy. Science, 346(6215), 1377-1380.
Russo, C. J., & Passmore, L. A. (2016). Ultrastable gold substrates: properties of a support for high-resolution electron cryomicroscopy of biological specimens. Journal of structural biology, 193(1), 33-44.
Passmore, L. A., & Russo, C. J. (2016). Specimen preparation for high-resolution cryo-EM. In Methods in enzymology (Vol. 579, pp. 51-86). Academic Press.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
687-200-AU | UltrAufoil, Regular Square Array of 2 m Diameter Holes – 2 m Separation, mounted on a 200M Au grid, package of 10 grids |
10 | € 395,40 | ||
688-300-AU | UltrAuFoil, Regular Square Array of 1.2 m Diameter Holes – 1.3 m Separation, mounted on a 300M Au grid, package of 10 grids |
10 | € 395,40 | ||
689-300-AU | UltrAuFoil, Regular Square Array of 0.6 m Diameter Holes – 1 m Separation, mounted on a 300M Au grid, package of 10 grids |
10 | p.o.r. | ||
687-200-AU-50 | UltrAufoil, Regular Square Array of 2 m Diameter Holes – 2 m Separation, mounted on a 200M Au grid, package of 50 grids |
50 | € 1.715,80 | ||
688-300-AU-50 | UltrAuFoil, Regular Square Array of 1.2 m Diameter Holes – 1.3 m Separation, mounted on a 300M Au grid, package of 50 grids |
50 | € 1.715,80 | ||
689-300-AU-50 | UltrAuFoil, Regular Square Array of 0.6 m Diameter Holes – 1 m Separation, mounted on a 300M Au grid, package of 50 grids |
50 | p.o.r. |