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Home / Accessories for Microscopy / Chemicals / Embedding / Embedding Components

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Embedding Components

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    Product
    Description
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    Product

    Polyvinyl Alcohol
    no. R1350

    Description

    Polyvinyl Alcohol molecular weight 30,000 – 70,000, 250g

    It is used as an alternative to methyl cellulose in cryo-ultramicrotomy.

    Ref: Tokuyasu, K.T. ‘The use of PVA pyrrolidone and PVA for cryo-ultramicrotomy’. Histochem J. 21, 163-171. 1989.

    Order

    Art.Description Unit PriceQuantity
    R1350

    Polyvinyl Alcohol Mol. Wt. 30,000 70,000

    250g p.o.r.
    Brand: Agar

    Product
    Description
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    Product

    OCT Compound
    Embedding medium for frozen tissue specimens.
    no. R1180

    Description

    OCT Compound 118ml

    Embedding medium for frozen tissue specimens.
    OCT Compound is a water-soluble blend of glycols and resins that provides a convenient specimen matrix for cryostat sectioning at temperatures of -10?C and below.
    OCT leaves no residue on slides during staining procedure, eliminating undesirable background staining.

    Dissected tissue, mounted in OCT Compound can be frozen between -20 and -80?C.
    Developed and optimized for chromogenic IHC experiments, OCT Compound will not dull microtome knives.

    OCT Compound contains:

    • Polyvinyl alcohol
    • Polyethylene glycol
    • Nonreactive ingredients

     

    Supplied in 118ml (4oz) squeeze bottles.

    Order

    Art.Description Unit PriceQuantity
    R1180

    OCT Compound

    118ml p.o.r.
    Brand: Agar

    Product
    Description
    Technical Note
    MSDS
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    Product

    Epoxy Resins
    Epoxy resins for the preparation and sectioning of specimens for TEM.
    no. R1040 to R1372

    Description

    Epoxy resins and methacrylates are often harmful. Resin blocks are harmless after curing but, if cured blocks are sawn, make sure that the dust is not inhaled.

    Agar 100 – replaces Epon 812 resin, now unobtainable. The formulation of Agar 100 resin is the same as Epon, but is less viscous than many samples of Epon 812 and is more closely controlled in its manufacture. Epoxide equivalent between 148 and 150.

    ERL 4221 has replaced ERL4206. It has a higher viscosity (180cps) than the previously used ERL 4206 (60cps). Ref: Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinylcyclohexene dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, Vol 14, No 4, July 2006.

    Technical Note

    Technical Note

    The original formulation, ERL 4206 (VCD) – DER® 736 embedding medium devised by Spurr (1969) combined very low viscosity components for rapid specimen penetration.

    This embedding medium has been especially suitable for a variety of mineral and biological specimens. Since ERL 4206 is no longer available, ERL 4221 has been substituted as a replacement*.

    It will work well for most users but it does have somewhat higher viscosity (180cp vs. 60cp) than the ERL 4206, which can impact some protocols employing vacuum embedding.

    *Reference: Ellis, E. Ann Microscopy Today, July, 33 (2006)

    A block of MEDIUM hardness can be obtained by using the following formula based on a batch of 10 grams without the flexibilizer, DER 736:

    Part no.DescriptionHardnessRapid
    MediumHarderSofter
    AGR1047RERL 42214.10 g
    AGR1072/73/74DER 736 Diglycidylether of Polypropyleneglycol1.43 g0.95 g1.90 g
    AGR1054/55/56NSA (Nonenyl Succinic Anhydride) redistilled5.90 g
    AGR1066/67S-1 (Dimethylaminoethanol)0.10 g0.20 g

     

    A harder block can be produced by decreasing the DER® 736 component to 0.95g in the above formula; a softer block by increasing the DER 736 to 1.90g.

    ALL STEPS MUST BE PERFORMED UNDER A HOOD AND GLOVES MUST BE WORN FOR PROTECTION.

    Preparation:

    Care should be taken to weigh the ingredients accurately into a beaker. The quantities given in the first column are for firm blocks.
    For harder blocks, decrease the DER 736 to 0.95g; for softer blocks, increase the DER 736 to 1.90g.

    For rapid cure, increase the DMAE. It is recommended to add the catalyst (dimethylaminoethanol) last, after having carefully mixed the other components.
    If bubbles are a problem, they may be eliminated by placing the beaker into a desiccator under a gentle vacuum.

    MSDS

    MSDS

    Download Material Safety Data Sheet – AGR1040, AGR1041 & AGR1042 Araldite Resin CY212
    Download Material Safety Data Sheet – AGR1043, AGR1044 & AGR1045 Agar 100 Resin
    Download Material Safety Data Sheet – AGR1370, AGR1371 & AGR1372 Low Viscosity Resin
    Download Material Safety Data Sheet – AGR1047R ERL-4221
    Order

    Art.Description Unit PriceQuantity
    R1040

    Araldite CY212 Resin

    500g p.o.r.
    R1041

    Araldite CY212 Resin

    250g p.o.r.
    R1042

    Araldite CY212 Resin

    100g p.o.r.
    R1043

    Agar 100 Resin

    500g p.o.r.
    R1044

    Agar 100 Resin

    250g p.o.r.
    R1045

    Agar 100 Resin

    100g p.o.r.
    R1047R

    ERL 4221

    225ml p.o.r.
    R1090

    Araldite RD-2 Resin

    500g p.o.r.
    R1091

    Araldite RD-2 Resin

    250g p.o.r.
    R1092

    Araldite RD-2 Resin

    100g p.o.r.
    R1370

    Agar Low Viscosity Resin

    500g p.o.r.
    R1371

    Agar Low Viscosity Resin

    250g p.o.r.
    R1372

    Agar Low Viscosity Resin

    100g p.o.r.
    Brand: Agar

    Product
    Description
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    Quetol 651
    This is an epoxy resin with a viscosity of 15 cps at 25 C and an epoxy equivalent of 115.
    no. R1068

    Description

    Quetol 651 500g

    This is an epoxy resin with a viscosity of 15 cps at 25 C and an epoxy equivalent of 115. It is miscible with water, alcohol and acetone.

    When used with NSA and DMP-30, it forms a low viscosity mix which penetrates tissue easily.

    Order

    Art.Description Unit PriceQuantity
    R1068

    Quetol 651

    500g p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Product

    Hardeners for Epoxy Resins
    Hardeners for epoxy resins
    no. R1051 to R1378

    Description

    Hardeners for epoxy resins

    DDSA, NSA + VH1 are irritants.
    MNA + VH2 are corrosive.

    MSDS

    MSDS

    Download Material Safety Data Sheet – AGR1051, AGR1052 & AGR1053 Dodecenylsuccinic Anhydridee
    Download Material Safety Data Sheet – AGR1054, AGR1055 & AGR1056 Nonenyl Succinic Anhydride
    Download Material Safety Data Sheet – AGR1081, AGR1082 & AGR1083 Methyl Nadic Anhydride
    Download Material Safety Data Sheet – AGR1373, AGR1374 & AGR1375 Low Viscosity Hardener
    Download Material Safety Data Sheet – AGR1376 Low Viscosity Hardener VH2
    Download Material Safety Data Sheet – AGR1377 Low Viscosity Hardener VH2
    Download Material Safety Data Sheet – AGR1378 Low Viscosity Hardener VH2
    Order

    Art.Description Unit PriceQuantity
    R1051

    DDSA (Dodecenyl Succinic Anhydride) EM grade redistilled

    500g p.o.r.
    R1052

    DDSA (Dodecenyl Succinic Anhydride) EM grade redistilled

    250g p.o.r.
    R1053

    DDSA (Dodecenyl Succinic Anhydride) EM grade redistilled

    100g p.o.r.
    R1054

    NSA (Nonenyl Succinic Anhydride) redistilled

    500g p.o.r.
    R1055

    NSA (Nonenyl Succinic Anhydride) redistilled

    250g p.o.r.
    R1056

    NSA (Nonenyl Succinic Anhydride) redistilled

    100g p.o.r.
    R1081

    MNA Methyl Nadic Anhydride redistilled

    500g p.o.r.
    R1082

    MNA Methyl Nadic Anhydride redistilled

    250g p.o.r.
    R1083

    MNA Methyl Nadic Anhydride redistilled

    100g p.o.r.
    R1373

    Low Viscosity Hardener VH1

    500g p.o.r.
    R1374

    Low Viscosity Hardener VH1

    250g p.o.r.
    R1375

    Low Viscosity Hardener VH1

    100g p.o.r.
    R1376

    Low Viscosity Hardener VH2

    500g p.o.r.
    R1377

    Low Viscosity Hardener VH2

    250g p.o.r.
    R1378

    Low Viscosity Hardener VH2

    100g p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Accelerators for Epoxy Resins
    BDMA is recommended in preference to DMP-30 since it is less viscous and has a longer shelf life.
    no. R1060 to R1381

    Description

    Accelerators for epoxy resins

    BDMA is recommended in preference to DMP-30 since it is less viscous and has a longer shelf life.

    Accelerators should be stored dry in a desiccator, but need not be in a refrigerator.
    All these materials have a shorter shelf life than the resins and should be discarded after six months.

    DMP-30 is harmful
    BDMA, S-1 + accelerator (LV) are corrosive.

    MSDS

    MSDS

    Download Material Safety Data Sheet – AGR1060, AGR1061 & AGR1062 BDMA Benzyldimethylamine
    Download Material Safety Data Sheet – AGR1063 DMP-30
    Download Material Safety Data Sheet – AGR1064 DMP-30
    Download Material Safety Data Sheet – AGR1065 DMP-30
    Download Material Safety Data Sheet – AGR1065 DMP-30 MSDS
    Download Material Safety Data Sheet – AGR1066 & AGR1067 S-1 2-Dimethylaminoethanol
    Download Material Safety Data Sheet – AGR1379, AGR1380 & AGR1381 LV accelerator
    Order

    Art.Description Unit PriceQuantity
    R1060

    BDMA Benzyl Dimethylamine

    500g p.o.r.
    R1061

    BDMA Benzyl Dimethylamine

    250g p.o.r.
    R1062

    BDMA Benzyl Dimethylamine

    100g p.o.r.
    R1063

    DMP-30 Tri-Dimethylaminomethyl Phenol

    500g p.o.r.
    R1064

    DMP-30 Tri-Dimethylaminomethyl Phenol

    250g p.o.r.
    R1065

    DMP-30 Tri-Dimethylaminomethyl Phenol

    100g p.o.r.
    R1066

    S-1 Dimethylaminoethanol

    500g p.o.r.
    R1067

    S-1 Dimethylaminoethanol

    100g p.o.r.
    R1379

    Low Viscosity Accelerator

    100ml p.o.r.
    R1380

    Low Viscosity Accelerator

    50ml p.o.r.
    R1381

    Low Viscosity Accelerator

    5 x 2,5ml p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Product

    Plasticisers
    Dibutyl Phthalate
    no. R1070 and R1071

    Description

    Dibutyl Phthalate Plasticisers

    MSDS

    MSDS

    Download Material Safety Data Sheet

     

    Order

    Art.Description Unit PriceQuantity
    R1070

    Dibutyl Phthalate

    500g p.o.r.
    R1071

    Dibutyl Phthalate

    100g p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Product

    Additives
    Additives - Epoxy. DER 736 is an irritant.
    no. R1072 to R1089

    Description

    DER 736 is an irritant.

    MSDS

    MSDS

    Download Material Safety Data Sheet
    Order

    Art.Description Unit PriceQuantity
    R1072

    DER 736 Diglycidylether of Polypropyleneglycol

    500g p.o.r.
    R1073

    DER 736 Diglycidylether of Polypropyleneglycol

    250g p.o.r.
    R1074

    DER 736 Diglycidylether of Polypropyleneglycol

    100g p.o.r.
    R1088

    N N-Dimethyl Aniline

    100g p.o.r.
    R1089

    2-Butoxyethanol

    500g p.o.r.
    Brand: Agar

    Product
    Description
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    Product

    Methyl Methacrylates
    Methyl methacrylate + n-Butyl meth are flammable.
    no. R1049 to R1086

    Description

    Methacrylates

    Methyl methacrylate + n-Butyl meth are flammable.
    All methacrylates are irritants.

    Order

    Art.Description Unit PriceQuantity
    R1049

    Methyl Methacrylate

    500g p.o.r.
    R1050

    N-Butyl Methacrylate

    500g p.o.r.
    R1084

    GMA (2-Hydroxyethyl Methacrylate)

    500g p.o.r.
    R1085

    GMA (2-Hydroxyethyl Methacrylate)

    250g p.o.r.
    R1086

    GMA (2-Hydroxyethyl Methacrylate)

    100g p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Product

    Polymerisation catalyst for methacrylates
    Polymerisation catalyst for methacrylates
    no. R1059 and R1058

    Description

    Damped with 25% water.

    MSDS

    MSDS

    Download Material Safety Data Sheet – Technical Note
    Order

    Art.Description Unit PriceQuantity
    AGR1059

    Benzoyl Peroxide

    25g € 27,60
    ✓
    spinner
    AGR1058

    Benzoyl Peroxide

    100g € 93,60
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    Brand: Agar

    Product
    Description
    Technical Note
    MSDS
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    Product

    Resin Solvents
    A solvent for unpolymerised or partially polymerised resins and their components.
    no. R1346 and R1347

    Description

    Resin solvents

    A solvent for unpolymerised or partially polymerised resins and their components.

    Clean glassware etc. with resin solvent and wash with water.
    The solvent is water miscible.
    An active solvent is also available for polymerised resins.

    Technical Note

    Technical Note

    Download Technical Note
    MSDS

    MSDS

    Download Material Safety Data Sheet – AGR1346 Solvent for Polymerised Resin
    Download Material Safety Data Sheet – AGR1347 Solvent for Unpolymerised Resin
    Order

    Art.Description Unit PriceQuantity
    R1347

    Solvent for Unpolymerised Resin

    500ml p.o.r.
    R1346

    Solvent for Polymerised Resin

    50ml p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Product

    Chemicals for Support Films
    A variety of film-forming solutions, including Formvar, Pioloform and Butvar, to prepare support films and replicas of smooth surfaces.
    no. R1201 to R1277

    Description

    A variety of film-forming solutions are available to prepare support films and replicas of smooth surfaces.

    Formvar (polyvinyl formal) is normally used as a 0.25% solution in chloroform.
    The thickness and stability of the film can be altered by adjusting the concentration between 0.25% and 0.5%.

    Pioloform is polyvinyl butyral dissolved in chloroform (0.3 – 1.5%).
    The solution should be stored in a refrigerator in an amber glass bottle, and brought up to room temperature before use.

    Butvar B98 is a polyvinyl butyral resin containing 20% polyvinyl alcohol, and is normally used as a 0.25% solution in chloroform.
    It is an alternative support film to Formvar as reported by: Handley and Olsen, Ultramicroscopy 4, p479 – 480, 1979.

    Films made from Butvar are hydrophilic, and are therefore suitable for negative staining methods, having good mechanical stability, high electron transparency and minimum intrinsic structure.

    Butvar B98 can also be applied to grids as an adhesive.

    MSDS

    MSDS

    Download Material Safety Data Sheet – AGR1201 & AGR1202 Formvar
    Download Material Safety Data Sheet – AGR1275 Pioloform
    Download Material Safety Data Sheet – AGR1276 Butvar
    Download Material Safety Data Sheet – AGR1277 Silicon Monoxide
    Order

    Art.Description Unit PriceQuantity
    R1201

    Formvar

    100g p.o.r.
    R1202

    Formvar

    25g p.o.r.
    R1275

    Pioloform Powder

    10g p.o.r.
    R1276

    Butvar B98 Powder

    25g p.o.r.
    R1277

    Silicon Monoxide

    10g p.o.r.
    Brand: Agar

    Product
    Description
    MSDS
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    Product

    Hexamethyldisilazane HMDS
    HMDS, [(CH3)3Si]2NH, can be used in place of critical point drying for the preparation of soft tissue for SEM examination, for example, of delicate insect tissues.
    no. R1228

    Description

    Hexamethyldisilazane (HMDS) 100ml

    HMDS, [(CH3)3Si]2NH, can be used in place of critical point drying for the preparation of soft tissue for SEM examination, for example, of delicate insect tissues.

    It is faster, preserves surface detail, reduces thermal and pressure stresses, and may also reduce the extraction of cellular components compared with CPD. HMDS can be used to dry specimens such as bacteria on polycarbonate filters. 100ml

    Bray, D.F. et al. ‘A comparison of HMDS, Peldri II, and critical drying methods for SEM of biological specimens’. Micros. Res. And Technique. 26, 489 – 495. 1993.

    MSDS

    MSDS

    Download Material Safety Data Sheet
    Order

    Art.Description Unit PriceQuantity
    R1228

    Hexamethyldisilazane HMDS

    100ml p.o.r.
    Brand: Agar

    Product
    Specifications
    MSDS
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    Product

    Indicating Silica Gel
    Indicating silica gel. 1kg.
    no. R1278A

    Specifications

    Specifications

    CAS Number7631-86-9
    pH1.5 to 5
    Molecular FormilaO2Si
    PackagingPlastic powder jar
    SMILESO=[Si]=O
    Molecular Weight (g/mol)60.083
    ChEBICHEBI:30563
    Physical FormSolid
    ColourBrown-Orange
    Quantity1kg
    MSDS

    MSDS

    Download Material Safety Data Sheet – Technical Note
    Order

    Art.Description Unit PriceQuantity
    R1278A

    Indicating Silica Gel

    1kg p.o.r.
    Brand: Agar

    Product
    Description
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    Product

    Paraffin Wax
    Embedding PARAFFIN wax histology 54-58°C.
    no.

    Description

    Paraffin Wax embedding is a technique used to infiltrate tissue samples with paraffin.
    The samples can then be sliced into thin sections for examination by light microscope or can be left in paraffin blocks in order to preserve them.

    Order

    Art.Description Unit PriceQuantity
    313110

    Embedding paraffin wax histology 54-58°c – high wax

    1kg € 5,83
    ✓
    spinner
    313110-20

    Embedding paraffin wax histology 54-58°c – high wax

    20kg € 112,32
    ✓
    spinner
    Brand: Emmonya

    Product
    Description
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    Product

    Paraplast Plus
    This double filtered paraffin embedding medium contains plastic polymers of regulated molecular weights and a small percentage (0.8 %) of dimethyl sulphoxide (DMSO)
    no. AGL4382

    Description

    Paraplast Plus 1kg

    Paraplast Plus is a double filtered paraffin embedding medium contains plastic polymers of regulated molecular weights and a small percentage (0.8 %) of dimethyl sulphoxide (DMSO) to penetrate tissues three times faster than standard media.

    It melts rapidly at 56 °C, and allows short ribbons of sections cut to 1 m, and excellent ribbon continuity with 2 m thicknesses.

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    Art.Description Unit PriceQuantity
    AGL4382

    Paraplast Plus

    1kg p.o.r.
    Brand: Agar

    Product
    Description
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    Product

    Polyester Wax
    Melting point 37 C.
    no. L4184

    Description

    Polyester Wax 500g

    Melting point 37 °C.

    Order

    Art.Description Unit PriceQuantity
    L4184

    Polyester Wax

    500g p.o.r.
    Brand: Agar

    Contact

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