The use of fluorescent labels and markers is widespread and immunofluorescence, which relies on the use of antibodies tagged with labels such as fluorescein, is a well-established technique. A common problem associated with the microscopical examination of these materials is that the illumination used for stimulating the fluorescence also causes degradation of the label and this causes fading of fluorescence. This is also a problem when trying to visualise materials having a low level of labelling. The photofading of labelled materials can be retarded by the use of the Citifluor mountants. They are also useful for rhodamine and DAPI.

The antifadent solutions AF1, 2 and 3 were specifically developed for use with FITC polyclonal antibodies but their use is now far more widespread. They show improved signal preservation with many other fluorochromes, including rhodamines and DAPI. AF4 contains the antifadent n-propyl gallate, which is particularly suitable for use with DAPI and Alexa dye-stained materials, as well as FITC-labelled materials

AF1
Glycerol phosphate buffered solution containing antifadent for use with labelled tissue sections and dead cells. The solution has a pH of approximately 10. Available in two sizes.

AF2
Glycerol solution containing antifadent for use with labelled tissue sections and dead cells. The solution has a pH of approximately 10. Available in two sizes.

AF3
Phosphate buffered saline solution containing antifadent for examination of whole live cells. The solution has a pH of approximately 10. Available in two sizes

AF4
Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells. An aqueous solution (75% AF4 and 25% water) has a pH of 5.

Cultures of purified cortical astrocytes with rabbit antisera against glial fibrillary acidic protein, a component of the astrocyte cytoskeleton. The rabbit antisera was visualised using fluorescein-conjugated sheep anti-rabbit 1 g antisera. Micrographs courtesy of Mark Noble, Institute of Neurology, London.

The first visual shows fluorescent using glycerol as the mountant media
The second visual shows fluorescent using Citifluor (Glycerol/PBS) as the mountant media

MSDS

Download Material Safety Data Sheet – R1320, R1320A Citifluor Glycerol Pbs Solution AF1

Download Material Safety Data Sheet – R1321, R1321A Citifluor Glycerol Solution AF2

Download Material Safety Data Sheet – GR1322, GR1322A Citifluor Phosphate Buffered Saline AF3

Download Material Safety Data Sheet – R1327 Citifluor AF4 Mountant Solution

AF100
Phosphate buffered saline solution containing antifadent, specially prepared for use with UVM 3 solution where fluorochrome fading is a problem. Only available in conjunction with an aqueous solution of poly(vinyl alcohol) or with UVM 1 and UVM 3.

AF200
Glycerol solution containing antifadent, specially prepared for use with UVM 2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dyes.

AF300
Glycerol solution containing antifadent, specially prepared for use with UVM 2 solution where fluorescein is the staining material. The solutions can also be used with other fluorochromes such as rhodamines etc

MSDS

Download Material Safety Data Sheet – R1328-1 Citifluor AF100 Antifadent Solution

Download Material Safety Data Sheet – R1328-2 Citifluor AF200 Antifadent Solution

Download Material Safety Data Sheet – R1328-3 Citifluor AF300 Antifadent Solution