Products
Cryo Embedding
Tissue Marking Dye
Our new tissue marking dyes are formulated to provide very bright and clear identification of microscopic areas of interest and to easily identify the orientation of excised tissue samples.
These dyes are readily visible on cut frozen tissue sections.
These dyes will not run, bleed, fade or lose intensity from tissue processing and/or during staining procedures in the histology laboratory.
- Permanent tissue marking dyes.
- Colors formulated to define margins without bleeding or fading.
- Durable, bright dyes hold permanently through processing and staining procedures.
- Unique lid design allows for complete removal or they may be conveniently flipped open with little risk of drying.
- Available in 2oz. flip-top bottles in the following colors: Yellow, Red, Green, Black, Blue
Applications/intended use:
- Mohs surgery procedures to mark tissue specimens to map and identify the orientation and borders of tissue specimens.
- Pathology grossing of tissue to identify areas of interest and orientation of surgically excised tissue specimens.
- Marking surgical margins.
- Identifying serial segments of large tissues to ensure proper orientation and embedding.
- Identification of multiple pieces of tissues processed in one cassette
- May be used on fresh or fixed tissue.
Note: Pretreatment of tissue to be dyed with #27208, ColorBond Mordant may greatly enhance the performance of tissue marking dyes, especially with fatty tissues.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27213 | Tissue marking dye |
5 | € 165,00 | ||
| 27213-1 | Tissue marking dye, yellow |
60g | € 38,10 | ||
| 27213-2 | Tissue marking dye, red |
60g | € 38,10 | ||
| 27213-3 | Tissue marking dye, green |
60g | € 38,10 | ||
| 27213-4 | Tissue marking dye, blue |
60g | € 38,10 | ||
| 27213-5 | Tissue marking dye, black |
60g | € 38,10 |
Tissue Marking Dye Starter Kit
Starter kit includes the following:
- Tissue Marking Dye Holding Rack #27213-R
- Five colors of Tissue Marking Dye #27213
- Dye Mordant #27208
- Four variations of dye applicators: 1,000 Cotton Tip #80907; 50 Polypropylene Spade Tip #81536; 50 of each Wide Foam Tip #81442 (1/2″ tip), #81444 13/16″ round tip)
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27213-RK | Tissue Marking Dye, 5 Color Kit, 2oz. flip-top bottles, includes ColorBond™ Dye Mordant Style 27208 and 4 types of dye applicators |
Each | € 344,20 |
PolarStat Plus
Medium viscosity formulation.
PolarStat Plus is an exceptional cryo embedding media that works well with an anti-roll plate or may be led off the blade with a brush.
It is especially well-suited for Mohs cryostat sectioning.
- Now available in 4 optimal colors to accompany the traditional white formulation.
- This formulation freezes at -10 C.
- Optimal cutting temperature ranges from below-10 C to -40 C.
- Does not produce a powder residue while sectioning at -25 C or colder.
- Works with Auto Cryo Approach in Microm cryostats.
- No residue is left on slides during the staining procedure, thereby eliminating undesirable background staining.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27224 | Polarstat plus cryo embedding |
Each | € 26,70 | ||
| 27301-1 | PolarStat Plus™ Cryo Embedding Media, 4oz. bottle, white white |
Each | € 29,20 | ||
| 27301-2 | PolarStat Plus™ Cryo Embedding Media, 4oz. bottle, blue blue |
Each | € 22,70 | ||
| 27301-3 | PolarStat Plus™ Cryo Embedding Media, 4oz. bottle, yellow yellow |
Each | € 22,80 | ||
| 27301-4 | PolarStat Plus™ Cryo Embedding Media, 4oz. bottle, green green |
Each | € 22,70 |
PELCO Cryo-Embedding Compound
PELCO Cryo-Embedding Compound is a water soluble glycol medium for use in embedding for frozen sections.
This low viscosity compound assures reliable freezing and smooth sections.
PELCO Cryo-Embedding Compound is a fully compatible, cost effective alternative to Product #27050, Tissue Tek O.C.T. Compound.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27300 | PELCO Cryo-Embedding Compound, 4 oz. |
Each | € 17,50 |
OCT Compound 118ml
Embedding medium for frozen tissue specimens.
OCT Compound is a water-soluble blend of glycols and resins that provides a convenient specimen matrix for cryostat sectioning at temperatures of -10?C and below.
OCT leaves no residue on slides during staining procedure, eliminating undesirable background staining.
Dissected tissue, mounted in OCT Compound can be frozen between -20 and -80?C.
Developed and optimized for chromogenic IHC experiments, OCT Compound will not dull microtome knives.
OCT Compound contains:
- Polyvinyl alcohol
- Polyethylene glycol
- Nonreactive ingredients
Supplied in 118ml (4oz) squeeze bottles.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1180 | OCT Compound |
118ml | p.o.r. |
Tissue Freezing Medium
Embedding matrix for frozen sections. Less curling, less ice artifacts, freezes faster.
118ml per bottle, case/12 bottles.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27209 | Tissue Freezing Medium |
118ml | € 48,40 |
PELCO Histo/Cyto-Freeze Cryo Spray
Excellent for cooling liquid, semi-solid and solid specimens.
Ideal for ultra-rapid freezing of fresh tissue, cooling of liquids to induce precipitation prior to filtering and cooling of field samples.
PELCO Histo/Cyto-Freeze delivers instant flash freezing for cryostat sectioning.
It cools down to -50 C in just three seconds.
To cut frozen sections, mount sample directly on the microtome holder, simply spray to flash freeze and section.
Time saving procedure and prevents water crystals built up caused by slower methods.
The provided nozzle delivers the freezing action exactly where it is needed.
Contains 300ml pure, 100% virgin tetrafluoroethane for enhanced freezing and cooling performance.
Non-flammable, non-corrosive odorless gas.
Ozone safe.
Contains no CFC’s.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27219 | PELCO Histo-Cyto-freeze™ |
300ml | € 36,70 |
Cryogen Spray
Meets federal environmental specifications.
Does not contain dichlorodifluoromethane, cools to -65 °C.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27218 | Cryogen Spray |
300ml | € 49,20 |
Cryo Microtome Lubrication Kit
Kit contains special low-temperature lubricants necessary for routine maintenance of cryostat microtomes.
Includes Oil “A”, Oil “B” and Grease “G”.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 27244 | Tissue-Tek Microtome Lubrication Kit |
Each | € 201,60 |
Pen for Frozen Sections: Fro-Tissuer
General Use: Cryo Sectioning
Surfaces: Glass
Adhesive pen for frozen sections.
Will prevent sections from falling off or wrinkling.
Effective with PAP/PAAP, ABC, LAB-SA and immunofluorescent methods.
Stable up to 110 °C.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 22302 | Pen for Frozen Sections |
Each | € 140,00 |
Tissue Capture Pen
General Use: Histology
Surfaces: Glass
one pen for two applications
The new Tissue Capture Pen for use with frozen or paraffin sections.
A coating of the glass slide will prevent the sections from falling off or wrinkling. Stable up to 110 °C.
Pen contains material for 3000-5000 applications.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 22310 | Tissue Capture Pen |
Each | € 147,60 |
SEAL N FREEZE BOX
The SEAL N FREEZE BOX is intended for the cryogenic freezing (also known as snap or flash freezing) of animal tissue samples for use in cryostat sectioning.
Maximize your efficiency by using the SEAL N FREEZECRYOTRAY with the compact SEAL N FREEZE BOX, which allows for convenient hands-free freezing.
The SEAL N FREEZE BOX allows users to freeze up to four samples simultaneously when used with SEAL N FREEZE CRYOTRAY inserts.
The box s stainless steel case and foam lining ensures optimal temperature control throughout the freezing process. Its small, compact size ensures easy storage.
For the freezing media, liquid nitrogen or a slurry of dry ice and alcohol may be used in the SEAL N FREEZE BOX.
Warnings:
- Only properly trained users should handle cryogenic freezing media such as liquid nitrogen and dry ice.
- Use the SEAL N FREEZE BOX on a level surface only to prevent tipping.
- Never allow the freezing media to come in contact with skin. Ensure that the proper Cryo Safety Gear is used when handling the freezing media:
- cryo gloves
- apron
- safety glasses or face shield.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 20956 | SEAL’N FREEZE BOX |
Each | € 251,00 |
Cryo-Gel embedding medium, 120g
Cryo-Gel is a highly viscous, water-soluble embedding medium for frozen sections.
The gel is packaged in a pump-dispenser bottle that can instantly dispense the gel in any position.
The dispenser has a built-in ‘guillotine’ that cleanly severs the gel from the pump spouts and prevents any stringy medium from pulling away when the bottle is removed.
User Directions: Simply dispense gel on a room temperature block-holder. Use a cold heat extractor to form a base layer.
Dispense additional gel.
Place the tissue on top of the gel.
Use a cold heat extractor to freeze tissue and form a block.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| C350 | Cryo-Gel, 120g |
Each | p.o.r. |
CytoFix fixative spray
Cytology fixative spray is designed for slide based cytology specimens for cellular preservation and transport. It is water soluble and contains no chlorofluorocarbons.
Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.
CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining.
The Cytofix will not cloud or obscure the specimen and allows safe storing or transporting of slides.
Note: Allow specimens to dry before sending to lab or for storage.
Available in pump bottle or aerosol can, dries in 4 to 6 minutes.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| C351 | CytoFix Spray (Non-Aerosol, pump bottle) |
110ml | p.o.r. | ||
| C352 | CytoFix Spray (Aerosol, can) |
110ml | p.o.r. |
Tissue-Tek Cryomold Moulds
Disposable plastic Cryomold moulds create a uniformly shaped, flat-surface specimen block when used with OCT compound for frozen sectioning.
The standard mould fits directly into Cryobar freezing wells of the Tissue Tek II microtome/cryostat.
The moulds are available in five sizes:
- Biopsy Cryomold 10 x 10 x 5mm
- Intermediate Cryomold 15 x 15 x 5mm
- Standard Cryomold 25 x 20 x 5mm
- Round Cryomold Standard 23mm
- Round Cryomold Standard 15mm
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| G4580 | Cryomold Standard 25 x 20 x 5mm |
100 | p.o.r. | ||
| G4581 | Cryomold Biopsy 10x10x5mm |
100 | p.o.r. | ||
| G4582 | Cryomold Intermediate 15x15x5mm |
100 | p.o.r. | ||
| G4583 | Round Cryomold Standard |
100 | p.o.r. | ||
| G4584 | Round Cryomold Biopsy |
100 | p.o.r. |
SEAL’N FREEZE CRYOTRAY Molds
The SEAL N FREEZE CRYOTRAY is a compact system of plastic cryo embedding molds with attached sealable lids.
This tray system allows for batch processing of tissue samples in perfect uniform blocks that are ideal for cryo sectioning and storage.
Each disposable plastic SEAL N FREEZE CRYOTRAY includes four individual molds which can be easily separated from the center connector by folding and tearing along the perforated borders.
SEAL N FREEZE CRYOTRAY molds are available in two sizes:
Intermediate: 15 L x 15 W x 5mm D, pkg/50 trays (200 molds)
Large: 25 L x 30 W x 20mm D, pkg/50 trays (200 molds)
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| 20956-1 | SEAL’N FREEZE CRYOTRAY Molds, Intermediate, 15 L x 15 W x 5mm D (0.6 L x 0.6 W x 0.2″ D) |
50 | € 126,10 | ||
| 20956-2 | SEAL’N FREEZE CRYOTRAY Molds, Large, 25 L x 30 W x 20mm D (1 L x 1.2 W x 0.8″ D) |
50 | € 124,40 |
LV CryoOil low viscosity oil, 1.5ml Vial
LV CryoOil Is the best solution to your oil needs. It has the lowest viscosity of any available perfluoropolyether oil (1/10 that of Paratone oil and compatible to that of vegetable oil) and very low surface tension (less than 1/3 that of water).
Oils are widely used as cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation, that may occur in air near and above room temperature.
The ice ring seen in the diffraction pattern of flash cooled protein crystals arise primarily from crystallization of the aqueous solution surrounding the crystal, not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants.
These problems are especially acute for the smallest crystals. When mineral oil, Paratone Oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more, and can contribute excess drag that increases sample motion in a cryostream.
For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.
Consequently, a dip in this oil followed by gentle tapping to shake off excess can yield protective oil films on your crystals of as little as 10 m thick.
LV CryoOil has extremely low vapor pressure, excellent chemical inert and excellent thermal stability.
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| C353 | LV CryoOil |
15ml | p.o.r. |
