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Stains for LM

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Stains for LM

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Acridine Orange
Methachromatic fluorescent cationic dye which permeates the cell membrane and intercalates DNA and RNA
no. R1701

Description

Acridine Orange is a methachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA.
It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Acridine Orange is predominantly used in fluorescence and epifluoresence microscopy.

Suitable for:

  • Detection of nucleic acids separated by gel electrophoresis
  • Fluorescence and epifluorescence microscopy
  • Analysis of mitochondria and lysosomes by flow cytometry
  • DNA staining

 

25g. Harmful.

CI number: 46005.

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R1701

Acridine Orange

 25g € 281,20
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Alcian Blue 8GX
Heteroglycan stain for mucosubstances
no. R1702

Description

Alcian Blue 8GX is used as a heteroglycan stain for mucosubstances including proteoglycans, glycoproteins and acetic mucins.

Harmful.

CI number: 74240

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R1702

Alcian Blue 8GX

 25g € 258,40
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Product
Alizarin Red
Anthraquinone derivative
no. R1729

Description

Alizarin Red is an anthraquinone derivative.
It is used to identify calcium in bone and tissue sections.

Alizarin Red will also detect magnesium, manganese, barium, strontium and iron.

25g. Harmful.
CI number: 58005

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R1729

Alizarin Red

 25g € 69,80
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Product
Aniline Blue
Fluorescent stain for glycogen. Alcohol soluble or water soluble
no. R1703 and R1704

Description

Fluorescent stain for glycogen.

Aniline Blue can also be used to stain collagen fibres in tissue sections using Masson’s trichrome protocol for staining multiple components.
While using this method, collagen is stained blue.

25g. Harmful.

Aniline Blue (alcohol soluble) CI number: 42775
Aniline Blue (water soluble) CI number: 42755

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Download Material Safety Data Sheet – R1704 Aniline Blue, Water Soluble
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Art.Description Unit PriceQuantity
R1704

Aniline Blue (Alcohol Soluble)

 25g € 100,40
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R1703

Aniline Blue (Water Soluble)

 25g € 71,20
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Product
Azur II
Stain for nuclei, blood and bone marrow smears.
no. R1705

Description

Azur II is a dry dye for the preparation of colour staining solution used for the staining of blood and bone marrow smears of human origin.

The dye has a constant dye content and a reliable high quality, which is one of the major preconditions for reproducible diagnostic staining.

Azur II can also be used to stain nuclei, goblet cells, striated muscle, endothelial cells, smooth muscle, cytoplasm, liquid droplets, red blood cells, cartilage, mucus granules, elastin and leukocytes.

Corrosive, harmful.

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R1705

Azur Il

 25g € 95,50
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Product
Brilliant Cresyl Blue
Supravital haematology stain for counting the reticulocytes in peripheral blood smears.
no. R1730

Description

Brilliant Cresyl Blue is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD).
This enzyme is generated in growing oocytes. However, oocytes that complete their growth have low levels of G6PD.

Low G6PD activity results in the blue colouration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound.

It is also used for the staining in reticulocytes, where the dye stains reticulin.

Certified as a supravital stain for reticulocytes.

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains an 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Harmful.

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R1730

Brilliant Cresyl Blue

 25g € 90,50
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Product
Brilliant Green
Basic dye which is soluble in both water and alcohol.
no. R1731

Description

Brilliant green is a basic dye which is soluble in both water and alcohol. It is often used as a counterstain alongside either Eosin Y or Safranine O.
It is primarily used to stain spores green whilst the cell elements remain red when using the Rakette method f spore staining.

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R1731

Brilliant Green

 25g € 208,20
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Product
Carmine
Red-coloured stain for animal starches such as glycogen.
no. R1732

Description

Carmine is a red-coloured dry dye that is used for the preparation of a staining solution for staining nuclei and for the demonstration of glycogen in histological sections of human origin.

Toxic.

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R1732

Carmine

 25g € 207,80
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Product
Congo Red
Metachromatic stain that is used to stain amyloid tissues.
no. R1733

Description

Congo Red is a metachromatic stain that is used to stain amyloid tissues.
Staining using Congo Red is intensified by the pre-treatment of tissue with alkaline sodium chloride.

Health hazard

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R1733

Congo Red

 25g € 61,50
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Product
Crystal Ponceau
Crystal Ponceau is predominantly used in the MSB staining procedure for the staining of connective tissue, fibrin, collagen fibres and erythrocytes.
no. R1708

Description

Crystal Ponceau is mainly used in the MSB (martius yellow-crystal scarlet-methyl blue) staining procedure for the staining of connective tissue, fibrin, collagen fibres and erythrocytes.

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Art.Description Unit PriceQuantity
R1708

Crystal Ponceau

 25g € 75,00
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Product
Eosin
Brominated fluorescein dye derivative used as an acidic red stain for cell cytoplasm. Yellowish (water soluble) or bluish (spirit soluble).
no. R1709

Description

Eosin is a brominated fluorescein dye derivative used as an acidic red stain for cell cytoplasm. It is a counterstain to haemotoxylin and colours red blood cells, cytoplasmic material, cell membranes and extracellular structures pink or red.

Available either as water soluble (Eosin Y – yellowish) or spirit soluble (Eosin B – bluish).

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Download Material Safety Data Sheet – R1709 Eosin Y (yellowish)
Download Material Safety Data Sheet – R1710 Eosin B (bluish)
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Art.Description Unit PriceQuantity
R1709

Eosin Y (yellowish – Water Soluble)

 25g € 129,70
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R1710

Eosin B (bluish – Spirit Soluble)

 25g € 130,90
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Product
Fast Green FCF
Tissue and protein stain and a quantitative stain for histone at alkaline pH after acid extraction of DNA.
no. R1711

Description

Fast Green FCF is a tissue and protein stain and a quantitative stain for histone at alkaline pH after acid extraction of DNA.
It can be used as a protein stain in IEF (Isoelectric Focusing) and can be used in SDS-PAGE electrophoresis.

Harmful, health hazard.

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R1711

Fast Green FCF

 25g € 210,20
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Product
Fluorescein
Stain for epithelial defects.
no. R1712

Description

Fluorescein is used to stain epithelial defects and analyse the integrity of cornelial epithelium.

Irritant.

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R1712

Fluorescein

 25g € 84,00
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Product
Acid Fuchsin
Stain for collagen, smooth muscle or mitochondria.
no. R1700

Description

Acid Fuchsin is used for staining collagen, smooth muscle or mitochondria.

Harmful.
CI Number: 42685

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Art.Description Unit PriceQuantity
R1700

Acid Fuchsin

 25g € 72,90
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Product
Ponceau de Xylidine
Poncaeu de Xylidine is used as a substitute for cochineal dye.
no. R1722

Description

Ponceau de Xylidine is used as a substitute for cochineal dye.

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R1722

Ponceau de Xylidine

 25g € 106,90
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Product
Light Green SF
Cytological counter stain.
no. R1716

Description

Light Green SF is used as a cytological counter stain and can be used to stain collagen fibres.

Harmful, health hazard.

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R1716

Light Green SF

 25g € 201,10
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Product
Pyronin Y
Pyronin Y is an intercalating cationic dye specifically for RNA.
no. R1723

Description

Pyronin Y is an intercalating cationic dye specifically for RNA.
It is visible as a red band during electrophoresis and is shown to accumulate in the mitochondria of viable cells.

Pyronin Y can be used in combination with Alcian Blue to assess and study pulmonary diseases and can also be used with Methyl Green to stain nucleic acids in paraffin sections.

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R1723

Pyronin Y

 10g € 325,00
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Product
Luxol Fast Blue MBS
Stain for myelinated axons in brain and spinal cord tissue, neurons, myelin and phospholipids.
no. R1717

Description

Luxol Fast Blue MBS is used to stain myelinated axons in brain and spinal cord tissue blue, neurons violet and myelin and phospholipids blue or green.

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R1717

Luxol Fast Blue MBS

 25g € 90,50
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Product
Methylene Blue
Stain for mitochondria, a variety of bacteria and leukocytes.
no. R1719

Description

Methylene Blue is used to stain animal stains to make the mitochondria more visible.
It can also be used to stain a variety of bacteria and leukocytes.

Corrosive, flammable, harmful.
CI Number: 52015

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R1719

Methylene Blue

 25g € 71,70
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Product
Safranin O
Safranin O is a nuclear stain used as a counter stain or to colour collagen yellow.
no. R1724

Description

Safranin O is a nuclear stain used as a counter stain or to colour collagen yellow.
It can also be used to stain mucin, cartilage and mast cell granules.

Safranin O stain, imaged by Kevin Mackenzie, University of Aberdeen.

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R1724

Safranin O

 25g € 170,10
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Product
Methyl Blue
Acid triphenylmethane dye which colours collagen fibres.
no. R1734

Description

Methyl Blue is an acid triphenylmethane dye and colours collagen fibres.

Harmful.
CI number: 42780

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R1734

Methyl Blue

 25g € 194,20
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Scarlet R (Sudan Iv)
This stain is a hydrophobic azo dye which is used to colour nonpolar substances.
no. R1737

Description

This stain is a hydrophobic azo dye which is used to colour nonpolar substances such as oils, fats, waxes, greases, various hydrocarbon products and acrylic emulsions.

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R1737

Scarlet R (Sudan Iv)

 25g € 29,90
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Product
Sudan Black B
Sudan Black B is a fat soluble diazo dye and can be used to stain neutral triglycerides and lipids.
no. R1725

Description

Sudan Black B is a fat soluble diazo dye and can be used to stain neutral triglycerides and lipids.
It is useful for detecting fats that have been contaminated with oil or grease and can be used to study haematological disorders.

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R1725

Sudan Black B

 25g € 101,20
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Product
Methyl Green
Chromogenic counterstain which stains nuclei acids green.
no. R1718

Description

Methyl green is a chromogenic counterstain and stains nuclei acids green.

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R1718

Methyl Green

 25g € 63,30
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Product
Tartrazine
Tartrazine is an anionic, hydrophilic azo dye with an orange-yellow colour.
no. R1726

Description

Tartrazine is an anionic, hydrophilic azo dye with an orange-yellow colour which is used in fabrics, foods and cosmetics, and also as a histological stain.
Tartrazine and Phloxine (red) to show Paneth cells in gut.

Imaged by Kevin McKenzie, University of Aberdeen.

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R1726

Tartrazine

 25g € 27,00
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Product
Neutral Red
Stains nuclei red, can be used as a histology stain for lysosomes and can also be used as a pH indicator.
no. R1720

Description

Neutral Red stains nuclei red.

It can be used as a histology stain for lysosomes and can also be used as a pH indicator.

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R1720

Neutral Red

 25g € 64,30
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Toluidine Blue
Toluidine Blue is a metachromatic dye used in many histological staining procedures.
no. R1727

Description

Toluidine Blue is a metachromatic dye used in many histological staining procedures.
It predominantly stains nucleic acids blue and polysaccharides purple.

Toluidine Blue stain, imaged by Kevin Mackenzie, University of Aberdeen

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R1727

Toluidine Blue

 10g € 41,50
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Product
Nigrosine (Water Soluble)
Acidic stain used for negative staining.
no. R1735

Description

Nigrosine (Water Soluble) is an acidic stain used for negative staining.
It is useful for studying the morphological shape, size and arrangement of bacteria cells that are too difficult to stain or are too delicate to be heat-fixed.

Uses for this stain in microscopy include viewing:

  • Bacteria and bacterial flagella
  • Viruses
  • Membrane structures
  • Proteins
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R1735

Nigrosine (Water Soluble)

 25g € 68,70
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Product
Van Gieson’s Stain
Counterstain specifically for collagen.
no. R1728

Description

Van Gieson’s stain is a counterstain that is specific for collagen, comprised of two acid dyes – piric acid and acid fuchsen.
It used to differentiate collagen and other connective tissue, particularly highlighting elastic fibres.

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R1728

Van Gieson s Stain

 500ml € 65,90
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Product
Oil Red O
Stain for lipids and fat.
no. R1736

Description

Oil Red O is used to stain lipids and fat.
It can also be used as a lysochrome diazo dye for staining neutral triglycerides and lipids on frozen sections, and some lipoproteins on paraffin sections.

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R1736

Oil Red O

 25g € 42,10
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Product
Orange G
Orange G is used to stain specimens for carcinoma diagnosis.
no. R1721

Description

Orange G is used to stain specimens for carcinoma diagnosis and is also used to in Papanicolaou’s stain technique in cytology.

Image from Kevin MacKenzie, University of Aberdeen: Section of human skin stained with aldehyde fuchsin, Orange G and Light Green histology dyes to highlight elastic fibres (purple) and collagen (green to yellow).

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R1721

Orange G

 25g € 93,30
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