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Stains for LM
- Apertures
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Acridine Orange is a methachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA.
It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.
Acridine Orange is predominantly used in fluorescence and epifluoresence microscopy.
Suitable for:
- Detection of nucleic acids separated by gel electrophoresis
- Fluorescence and epifluorescence microscopy
- Analysis of mitochondria and lysosomes by flow cytometry
- DNA staining
25g. Harmful.
CI number: 46005.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1701 | Acridine Orange |
25g | € 281,20 |
Alcian Blue 8GX is used as a heteroglycan stain for mucosubstances including proteoglycans, glycoproteins and acetic mucins.
Harmful.
CI number: 74240
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1702 | Alcian Blue 8GX |
25g | € 258,40 |
Alizarin Red is an anthraquinone derivative.
It is used to identify calcium in bone and tissue sections.
Alizarin Red will also detect magnesium, manganese, barium, strontium and iron.
25g. Harmful.
CI number: 58005
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1729 | Alizarin Red |
25g | € 69,80 |
Fluorescent stain for glycogen.
Aniline Blue can also be used to stain collagen fibres in tissue sections using Masson’s trichrome protocol for staining multiple components.
While using this method, collagen is stained blue.
25g. Harmful.
Aniline Blue (alcohol soluble) CI number: 42775
Aniline Blue (water soluble) CI number: 42755
MSDS
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Download Material Safety Data Sheet – R1703 Aniline Blue, Alcohol Soluble |
![]() |
Download Material Safety Data Sheet – R1704 Aniline Blue, Water Soluble |
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1704 | Aniline Blue (Alcohol Soluble) |
25g | € 100,40 | ||
R1703 | Aniline Blue (Water Soluble) |
25g | € 71,20 |
Azur II is a dry dye for the preparation of colour staining solution used for the staining of blood and bone marrow smears of human origin.
The dye has a constant dye content and a reliable high quality, which is one of the major preconditions for reproducible diagnostic staining.
Azur II can also be used to stain nuclei, goblet cells, striated muscle, endothelial cells, smooth muscle, cytoplasm, liquid droplets, red blood cells, cartilage, mucus granules, elastin and leukocytes.
Corrosive, harmful.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1705 | Azur Il |
25g | € 95,50 |
Brilliant Cresyl Blue is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD).
This enzyme is generated in growing oocytes. However, oocytes that complete their growth have low levels of G6PD.
Low G6PD activity results in the blue colouration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound.
It is also used for the staining in reticulocytes, where the dye stains reticulin.
Certified as a supravital stain for reticulocytes.
The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains an 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.
Harmful.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1730 | Brilliant Cresyl Blue |
25g | € 90,50 |
Brilliant green is a basic dye which is soluble in both water and alcohol. It is often used as a counterstain alongside either Eosin Y or Safranine O.
It is primarily used to stain spores green whilst the cell elements remain red when using the Rakette method f spore staining.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1731 | Brilliant Green |
25g | € 208,20 |
Carmine is a red-coloured dry dye that is used for the preparation of a staining solution for staining nuclei and for the demonstration of glycogen in histological sections of human origin.
Toxic.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1732 | Carmine |
25g | € 207,80 |
Congo Red is a metachromatic stain that is used to stain amyloid tissues.
Staining using Congo Red is intensified by the pre-treatment of tissue with alkaline sodium chloride.
Health hazard
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1733 | Congo Red |
25g | € 61,50 |
Crystal Ponceau is mainly used in the MSB (martius yellow-crystal scarlet-methyl blue) staining procedure for the staining of connective tissue, fibrin, collagen fibres and erythrocytes.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1708 | Crystal Ponceau |
25g | € 75,00 |
Eosin is a brominated fluorescein dye derivative used as an acidic red stain for cell cytoplasm. It is a counterstain to haemotoxylin and colours red blood cells, cytoplasmic material, cell membranes and extracellular structures pink or red.
Available either as water soluble (Eosin Y – yellowish) or spirit soluble (Eosin B – bluish).
MSDS
![]() |
Download Material Safety Data Sheet – R1709 Eosin Y (yellowish) |
![]() |
Download Material Safety Data Sheet – R1710 Eosin B (bluish) |
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1709 | Eosin Y (yellowish – Water Soluble) |
25g | € 129,70 | ||
R1710 | Eosin B (bluish – Spirit Soluble) |
25g | € 130,90 |
Fast Green FCF is a tissue and protein stain and a quantitative stain for histone at alkaline pH after acid extraction of DNA.
It can be used as a protein stain in IEF (Isoelectric Focusing) and can be used in SDS-PAGE electrophoresis.
Harmful, health hazard.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1711 | Fast Green FCF |
25g | € 210,20 |
Fluorescein is used to stain epithelial defects and analyse the integrity of cornelial epithelium.
Irritant.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1712 | Fluorescein |
25g | € 84,00 |
Acid Fuchsin is used for staining collagen, smooth muscle or mitochondria.
Harmful.
CI Number: 42685
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1700 | Acid Fuchsin |
25g | € 72,90 |
Ponceau de Xylidine is used as a substitute for cochineal dye.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1722 | Ponceau de Xylidine |
25g | € 106,90 |
Light Green SF is used as a cytological counter stain and can be used to stain collagen fibres.
Harmful, health hazard.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1716 | Light Green SF |
25g | € 201,10 |
Pyronin Y is an intercalating cationic dye specifically for RNA.
It is visible as a red band during electrophoresis and is shown to accumulate in the mitochondria of viable cells.
Pyronin Y can be used in combination with Alcian Blue to assess and study pulmonary diseases and can also be used with Methyl Green to stain nucleic acids in paraffin sections.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1723 | Pyronin Y |
10g | € 325,00 |
Luxol Fast Blue MBS is used to stain myelinated axons in brain and spinal cord tissue blue, neurons violet and myelin and phospholipids blue or green.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1717 | Luxol Fast Blue MBS |
25g | € 90,50 |
Methylene Blue is used to stain animal stains to make the mitochondria more visible.
It can also be used to stain a variety of bacteria and leukocytes.
Corrosive, flammable, harmful.
CI Number: 52015
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1719 | Methylene Blue |
25g | € 71,70 |
Safranin O is a nuclear stain used as a counter stain or to colour collagen yellow.
It can also be used to stain mucin, cartilage and mast cell granules.
Safranin O stain, imaged by Kevin Mackenzie, University of Aberdeen.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1724 | Safranin O |
25g | € 170,10 |
Methyl Blue is an acid triphenylmethane dye and colours collagen fibres.
Harmful.
CI number: 42780
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1734 | Methyl Blue |
25g | € 194,20 |
This stain is a hydrophobic azo dye which is used to colour nonpolar substances such as oils, fats, waxes, greases, various hydrocarbon products and acrylic emulsions.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1737 | Scarlet R (Sudan Iv) |
25g | € 29,90 |
Sudan Black B is a fat soluble diazo dye and can be used to stain neutral triglycerides and lipids.
It is useful for detecting fats that have been contaminated with oil or grease and can be used to study haematological disorders.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1725 | Sudan Black B |
25g | € 101,20 |
Methyl green is a chromogenic counterstain and stains nuclei acids green.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1718 | Methyl Green |
25g | € 63,30 |
Tartrazine is an anionic, hydrophilic azo dye with an orange-yellow colour which is used in fabrics, foods and cosmetics, and also as a histological stain.
Tartrazine and Phloxine (red) to show Paneth cells in gut.
Imaged by Kevin McKenzie, University of Aberdeen.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1726 | Tartrazine |
25g | € 27,00 |
Neutral Red stains nuclei red.
It can be used as a histology stain for lysosomes and can also be used as a pH indicator.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1720 | Neutral Red |
25g | € 64,30 |
Toluidine Blue is a metachromatic dye used in many histological staining procedures.
It predominantly stains nucleic acids blue and polysaccharides purple.
Toluidine Blue stain, imaged by Kevin Mackenzie, University of Aberdeen
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1727 | Toluidine Blue |
10g | € 41,50 |
Nigrosine (Water Soluble) is an acidic stain used for negative staining.
It is useful for studying the morphological shape, size and arrangement of bacteria cells that are too difficult to stain or are too delicate to be heat-fixed.
Uses for this stain in microscopy include viewing:
- Bacteria and bacterial flagella
- Viruses
- Membrane structures
- Proteins
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1735 | Nigrosine (Water Soluble) |
25g | € 68,70 |
Van Gieson’s stain is a counterstain that is specific for collagen, comprised of two acid dyes – piric acid and acid fuchsen.
It used to differentiate collagen and other connective tissue, particularly highlighting elastic fibres.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1728 | Van Gieson s Stain |
500ml | € 65,90 |
Oil Red O is used to stain lipids and fat.
It can also be used as a lysochrome diazo dye for staining neutral triglycerides and lipids on frozen sections, and some lipoproteins on paraffin sections.
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1736 | Oil Red O |
25g | € 42,10 |
Orange G is used to stain specimens for carcinoma diagnosis and is also used to in Papanicolaou’s stain technique in cytology.
Image from Kevin MacKenzie, University of Aberdeen: Section of human skin stained with aldehyde fuchsin, Orange G and Light Green histology dyes to highlight elastic fibres (purple) and collagen (green to yellow).
Art. | Description | Unit | Price | Quantity | |
---|---|---|---|---|---|
R1721 | Orange G |
25g | € 93,30 |