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Home / Accessories for Microscopy / Chemicals / Stains / Stains for LM

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Stains for LM

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    Acridine Orange
    Methachromatic fluorescent cationic dye which permeates the cell membrane and intercalates DNA and RNA
    no. R1701

    Description

    Acridine Orange is a methachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA.
    It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

    Acridine Orange is predominantly used in fluorescence and epifluoresence microscopy.

    Suitable for:

    • Detection of nucleic acids separated by gel electrophoresis
    • Fluorescence and epifluorescence microscopy
    • Analysis of mitochondria and lysosomes by flow cytometry
    • DNA staining

     

    25g. Harmful.

    CI number: 46005.

    MSDS

    MSDS

    Download Material Safety Data Sheet – Technical Note
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    Art.Description Unit PriceQuantity
    R1701

    Acridine Orange

    25g € 281,20
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    Brand: Agar

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    Alcian Blue 8GX
    Heteroglycan stain for mucosubstances
    no. R1702

    Description

    Alcian Blue 8GX is used as a heteroglycan stain for mucosubstances including proteoglycans, glycoproteins and acetic mucins.

    Harmful.

    CI number: 74240

    Order

    Art.Description Unit PriceQuantity
    R1702

    Alcian Blue 8GX

    25g € 258,40
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    Brand: Agar

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    Alizarin Red
    Anthraquinone derivative
    no. R1729

    Description

    Alizarin Red is an anthraquinone derivative.
    It is used to identify calcium in bone and tissue sections.

    Alizarin Red will also detect magnesium, manganese, barium, strontium and iron.

    25g. Harmful.
    CI number: 58005

    MSDS

    MSDS

    Download Material Safety Data Sheet
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    Art.Description Unit PriceQuantity
    R1729

    Alizarin Red

    25g € 69,80
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    Brand: Agar

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    Aniline Blue
    Fluorescent stain for glycogen. Alcohol soluble or water soluble
    no. R1703 and R1704

    Description

    Fluorescent stain for glycogen.

    Aniline Blue can also be used to stain collagen fibres in tissue sections using Masson’s trichrome protocol for staining multiple components.
    While using this method, collagen is stained blue.

    25g. Harmful.

    Aniline Blue (alcohol soluble) CI number: 42775
    Aniline Blue (water soluble) CI number: 42755

    MSDS

    MSDS

    Download Material Safety Data Sheet – R1703 Aniline Blue, Alcohol Soluble
    Download Material Safety Data Sheet – R1704 Aniline Blue, Water Soluble
    Order

    Art.Description Unit PriceQuantity
    R1704

    Aniline Blue (Alcohol Soluble)

    25g € 100,40
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    R1703

    Aniline Blue (Water Soluble)

    25g € 71,20
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    Brand: Agar

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    Azur II
    Stain for nuclei, blood and bone marrow smears.
    no. R1705

    Description

    Azur II is a dry dye for the preparation of colour staining solution used for the staining of blood and bone marrow smears of human origin.

    The dye has a constant dye content and a reliable high quality, which is one of the major preconditions for reproducible diagnostic staining.

    Azur II can also be used to stain nuclei, goblet cells, striated muscle, endothelial cells, smooth muscle, cytoplasm, liquid droplets, red blood cells, cartilage, mucus granules, elastin and leukocytes.

    Corrosive, harmful.

    MSDS

    MSDS

    Download Material Safety Data Sheet
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    Art.Description Unit PriceQuantity
    R1705

    Azur Il

    25g € 95,50
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    Brand: Agar

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    Brilliant Cresyl Blue
    Supravital haematology stain for counting the reticulocytes in peripheral blood smears.
    no. R1730

    Description

    Brilliant Cresyl Blue is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD).
    This enzyme is generated in growing oocytes. However, oocytes that complete their growth have low levels of G6PD.

    Low G6PD activity results in the blue colouration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound.

    It is also used for the staining in reticulocytes, where the dye stains reticulin.

    Certified as a supravital stain for reticulocytes.

    The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains an 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

    Harmful.

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    R1730

    Brilliant Cresyl Blue

    25g € 90,50
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    Brilliant Green
    Basic dye which is soluble in both water and alcohol.
    no. R1731

    Description

    Brilliant green is a basic dye which is soluble in both water and alcohol. It is often used as a counterstain alongside either Eosin Y or Safranine O.
    It is primarily used to stain spores green whilst the cell elements remain red when using the Rakette method f spore staining.

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    Brilliant Green

    25g € 208,20
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    Carmine
    Red-coloured stain for animal starches such as glycogen.
    no. R1732

    Description

    Carmine is a red-coloured dry dye that is used for the preparation of a staining solution for staining nuclei and for the demonstration of glycogen in histological sections of human origin.

    Toxic.

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    Carmine

    25g € 207,80
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    Congo Red
    Metachromatic stain that is used to stain amyloid tissues.
    no. R1733

    Description

    Congo Red is a metachromatic stain that is used to stain amyloid tissues.
    Staining using Congo Red is intensified by the pre-treatment of tissue with alkaline sodium chloride.

    Health hazard

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    R1733

    Congo Red

    25g € 61,50
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    Crystal Ponceau
    Crystal Ponceau is predominantly used in the MSB staining procedure for the staining of connective tissue, fibrin, collagen fibres and erythrocytes.
    no. R1708

    Description

    Crystal Ponceau is mainly used in the MSB (martius yellow-crystal scarlet-methyl blue) staining procedure for the staining of connective tissue, fibrin, collagen fibres and erythrocytes.

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    R1708

    Crystal Ponceau

    25g € 75,00
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    Eosin
    Brominated fluorescein dye derivative used as an acidic red stain for cell cytoplasm. Yellowish (water soluble) or bluish (spirit soluble).
    no. R1709

    Description

    Eosin is a brominated fluorescein dye derivative used as an acidic red stain for cell cytoplasm. It is a counterstain to haemotoxylin and colours red blood cells, cytoplasmic material, cell membranes and extracellular structures pink or red.

    Available either as water soluble (Eosin Y – yellowish) or spirit soluble (Eosin B – bluish).

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    R1709

    Eosin Y (yellowish – Water Soluble)

    25g € 129,70
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    R1710

    Eosin B (bluish – Spirit Soluble)

    25g € 130,90
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    Fast Green FCF
    Tissue and protein stain and a quantitative stain for histone at alkaline pH after acid extraction of DNA.
    no. R1711

    Description

    Fast Green FCF is a tissue and protein stain and a quantitative stain for histone at alkaline pH after acid extraction of DNA.
    It can be used as a protein stain in IEF (Isoelectric Focusing) and can be used in SDS-PAGE electrophoresis.

    Harmful, health hazard.

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    R1711

    Fast Green FCF

    25g € 210,20
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    Fluorescein
    Stain for epithelial defects.
    no. R1712

    Description

    Fluorescein is used to stain epithelial defects and analyse the integrity of cornelial epithelium.

    Irritant.

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    R1712

    Fluorescein

    25g € 84,00
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    Acid Fuchsin
    Stain for collagen, smooth muscle or mitochondria.
    no. R1700

    Description

    Acid Fuchsin is used for staining collagen, smooth muscle or mitochondria.

    Harmful.
    CI Number: 42685

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    R1700

    Acid Fuchsin

    25g € 72,90
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    Ponceau de Xylidine
    Poncaeu de Xylidine is used as a substitute for cochineal dye.
    no. R1722

    Description

    Ponceau de Xylidine is used as a substitute for cochineal dye.

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    R1722

    Ponceau de Xylidine

    25g € 106,90
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    Light Green SF
    Cytological counter stain.
    no. R1716

    Description

    Light Green SF is used as a cytological counter stain and can be used to stain collagen fibres.

    Harmful, health hazard.

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    R1716

    Light Green SF

    25g € 201,10
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    Pyronin Y
    Pyronin Y is an intercalating cationic dye specifically for RNA.
    no. R1723

    Description

    Pyronin Y is an intercalating cationic dye specifically for RNA.
    It is visible as a red band during electrophoresis and is shown to accumulate in the mitochondria of viable cells.

    Pyronin Y can be used in combination with Alcian Blue to assess and study pulmonary diseases and can also be used with Methyl Green to stain nucleic acids in paraffin sections.

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    R1723

    Pyronin Y

    10g € 325,00
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    Luxol Fast Blue MBS
    Stain for myelinated axons in brain and spinal cord tissue, neurons, myelin and phospholipids.
    no. R1717

    Description

    Luxol Fast Blue MBS is used to stain myelinated axons in brain and spinal cord tissue blue, neurons violet and myelin and phospholipids blue or green.

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    R1717

    Luxol Fast Blue MBS

    25g € 90,50
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    Methylene Blue
    Stain for mitochondria, a variety of bacteria and leukocytes.
    no. R1719

    Description

    Methylene Blue is used to stain animal stains to make the mitochondria more visible.
    It can also be used to stain a variety of bacteria and leukocytes.

    Corrosive, flammable, harmful.
    CI Number: 52015

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    R1719

    Methylene Blue

    25g € 71,70
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    Safranin O
    Safranin O is a nuclear stain used as a counter stain or to colour collagen yellow.
    no. R1724

    Description

    Safranin O is a nuclear stain used as a counter stain or to colour collagen yellow.
    It can also be used to stain mucin, cartilage and mast cell granules.

    Safranin O stain, imaged by Kevin Mackenzie, University of Aberdeen.

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    R1724

    Safranin O

    25g € 170,10
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    Methyl Blue
    Acid triphenylmethane dye which colours collagen fibres.
    no. R1734

    Description

    Methyl Blue is an acid triphenylmethane dye and colours collagen fibres.

    Harmful.
    CI number: 42780

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    R1734

    Methyl Blue

    25g € 194,20
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    Scarlet R (Sudan Iv)
    This stain is a hydrophobic azo dye which is used to colour nonpolar substances.
    no. R1737

    Description

    This stain is a hydrophobic azo dye which is used to colour nonpolar substances such as oils, fats, waxes, greases, various hydrocarbon products and acrylic emulsions.

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    R1737

    Scarlet R (Sudan Iv)

    25g € 29,90
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    Sudan Black B
    Sudan Black B is a fat soluble diazo dye and can be used to stain neutral triglycerides and lipids.
    no. R1725

    Description

    Sudan Black B is a fat soluble diazo dye and can be used to stain neutral triglycerides and lipids.
    It is useful for detecting fats that have been contaminated with oil or grease and can be used to study haematological disorders.

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    R1725

    Sudan Black B

    25g € 101,20
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    Methyl Green
    Chromogenic counterstain which stains nuclei acids green.
    no. R1718

    Description

    Methyl green is a chromogenic counterstain and stains nuclei acids green.

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    R1718

    Methyl Green

    25g € 63,30
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    Tartrazine
    Tartrazine is an anionic, hydrophilic azo dye with an orange-yellow colour.
    no. R1726

    Description

    Tartrazine is an anionic, hydrophilic azo dye with an orange-yellow colour which is used in fabrics, foods and cosmetics, and also as a histological stain.
    Tartrazine and Phloxine (red) to show Paneth cells in gut.

    Imaged by Kevin McKenzie, University of Aberdeen.

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    R1726

    Tartrazine

    25g € 27,00
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    Neutral Red
    Stains nuclei red, can be used as a histology stain for lysosomes and can also be used as a pH indicator.
    no. R1720

    Description

    Neutral Red stains nuclei red.

    It can be used as a histology stain for lysosomes and can also be used as a pH indicator.

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    R1720

    Neutral Red

    25g € 64,30
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    Toluidine Blue
    Toluidine Blue is a metachromatic dye used in many histological staining procedures.
    no. R1727

    Description

    Toluidine Blue is a metachromatic dye used in many histological staining procedures.
    It predominantly stains nucleic acids blue and polysaccharides purple.

    Toluidine Blue stain, imaged by Kevin Mackenzie, University of Aberdeen

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    R1727

    Toluidine Blue

    10g € 41,50
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    Nigrosine (Water Soluble)
    Acidic stain used for negative staining.
    no. R1735

    Description

    Nigrosine (Water Soluble) is an acidic stain used for negative staining.
    It is useful for studying the morphological shape, size and arrangement of bacteria cells that are too difficult to stain or are too delicate to be heat-fixed.

    Uses for this stain in microscopy include viewing:

    • Bacteria and bacterial flagella
    • Viruses
    • Membrane structures
    • Proteins
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    R1735

    Nigrosine (Water Soluble)

    25g € 68,70
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    Van Gieson’s Stain
    Counterstain specifically for collagen.
    no. R1728

    Description

    Van Gieson’s stain is a counterstain that is specific for collagen, comprised of two acid dyes – piric acid and acid fuchsen.
    It used to differentiate collagen and other connective tissue, particularly highlighting elastic fibres.

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    R1728

    Van Gieson s Stain

    500ml € 65,90
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    Oil Red O
    Stain for lipids and fat.
    no. R1736

    Description

    Oil Red O is used to stain lipids and fat.
    It can also be used as a lysochrome diazo dye for staining neutral triglycerides and lipids on frozen sections, and some lipoproteins on paraffin sections.

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    R1736

    Oil Red O

    25g € 42,10
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    Orange G
    Orange G is used to stain specimens for carcinoma diagnosis.
    no. R1721

    Description

    Orange G is used to stain specimens for carcinoma diagnosis and is also used to in Papanicolaou’s stain technique in cytology.

    Image from Kevin MacKenzie, University of Aberdeen: Section of human skin stained with aldehyde fuchsin, Orange G and Light Green histology dyes to highlight elastic fibres (purple) and collagen (green to yellow).

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    R1721

    Orange G

    25g € 93,30
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