Products
Stains for EM
Stains for electron microscopy
All stains are EM grade
| Ammonium molybdate | – | Irritant. Negative stain. |
| Indium trichloride | – | Corrosive. Metal stain for nucleic acids. |
| Lanthanum nitrate | – | Oxidising, irritant. Used to prepare colloidal lanthanum hydroxidecontaining fixatives for the demonstration of intercellular spaces. |
| Lead acetate. Toxic | – | Metal stain for TEM, used for in-block and thin section staining. |
| Lead citrate | – | Toxic. For the preparation of a simplified lead stain. The most widely used metal stain for ultrathin sections. Lead Citrate is a restricted chemical and can only be shipped within the EU. |
| Lead nitrate | – | Oxidising, toxic. Metal stain for ultrathin sections. |
| Methylamine Tungstate | – | 1g |
| Phosphomolybdic acid | – | Oxidising, corrosive. Positive and negative stain. |
| Phosphotungstic acid | – | Corrosive. Positive and negative stain. |
| Potassium pyroantimonate | – | Harmful |
| Ruthenium red | – | Positive stain. |
| Sodium silicotungstate | – | Negative stain. |
| Uranyl acetate | – | Very toxic, radioactive. Universal stain for thin sections, in-block staining and negative staining. |
Technical Note
Methylamine tungstate is an excellent negative stain.
Unlike phosphotungstic acid, it does not damage virus particles and is consequently valuable for staining delicate viruses.
Contrast is good, though not as good as that obtained with uranyl stains; the resolution is good, and the material wets grid films and specimens very well.
See Faberge A.C. and Olivier R.M., Microscopie 20, 242, 1974 for application to plant viruses.
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Download Technical Note |
MSDS
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Download Material Safety Data Sheet – AGR1156 Ammonium molybdate |
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Download Material Safety Data Sheet – AGR1209 Lead Acetate |
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Download Material Safety Data Sheet – AGR1213 Phosphotungstic Acid Hydrate |
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Download Material Safety Data Sheet – AGR1214 Potassium Pyroantimonate |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1155 | Ammonium Molybdate |
100g | p.o.r. | ||
| R1156 | Ammonium Molybdate |
25g | p.o.r. | ||
| R1205 | Indium Trichloride |
10g | p.o.r. | ||
| R1207 | Lanthanum Nitrate |
25g | p.o.r. | ||
| R1209 | Lead Acetate |
250g | p.o.r. | ||
| R1210 | Lead Citrate |
50g | p.o.r. | ||
| R1217 | Lead Nitrate |
500g | p.o.r. | ||
| R1212 | Phosphomolybdic Acid |
25g | p.o.r. | ||
| R1213 | Phosphotungstic Acid |
25g | p.o.r. | ||
| R1214 | Potassium Pyroantimonate |
100g | p.o.r. | ||
| R1226 | Ruthenium Red |
1g | p.o.r. | ||
| R1227 | Ruthenium Red |
100mg | p.o.r. | ||
| R1230 | Sodium Silicotungstate |
25g | p.o.r. | ||
| R1260A | Uranyl Acetate |
25g | p.o.r. |
We can offer three alternatives to Uranyl Acetate for territories where shipping of radioactive substances is prohibitive.
These alternatives to Uranyl Acetate are non radioactive EM grade universal stains.
UA-Zero is a patented solution developed as a direct substitute for Uranyl Acetate.
UA-Zero does not contain any radioactive material and is non-toxic.
It can be used as a direct replacement for Uranyl Acetate with no changes to standard user protocols and delivers high contrast imaging.
AGR1260D is a mixture of lanthanum, samarium and gadolinium salts.
Technical Note
EM Stain 336 is a mixture of lanthanum salts, samarium triacetate (Sm(CH3COO)3) and gadolinium triacetate Gd(CHVCOO)3).
Both are reasonably soluble in water showing a neutral pH at room temperature.
Whilst initial staining of sections may not quite equal that of uranyl salts, post staining with osmium tetroxide before embedding increases the stain density dramatically.
Standard protocols for specimen fixation can be followed but best results will be attained if post fixation using 1% osmium tetroxide (OsO4) is undertaken before embedding and staining.
Method for Staining Sections
Dilute the original EM Stain 336 4 x with distilled water.
Place drops of the diluted stain on a dental wax sheet and invert the grids containing the sections onto the drops. Staining time is normally around 30 minutes at room temperature but can be shortened or extended according to section requirements.
Grids should be washed preferably by running distilled water down between the tines of the handling tweezers otherwise grids should be dipped at least three times in fresh distilled water.
MSDS
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Download Material Safety Data Sheet – AGR1000 UA-Zero EM Stain |
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Download Material Safety Data Sheet – AGR1260C Platinum Blue Uranyl Acetate Alternative |
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Download Material Safety Data Sheet – AGR1260D EM Stain 336 Uranyl Acetate Alternative |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1260D | EM Stain 336 Uranyl Acetate Alternative |
25ml | p.o.r. | ||
| R1000 | UA-Zero EM Stain |
25ml | p.o.r. |
