Products
Embedding kits
Glycol Methacrylate Resin Kit
The kit consists of:
- GMA (2-Hydroxyethyl methacrylate). 5 x 100 g Irritant
- 2-Butoxyethanol. 100 g Harmful
- Carbowax 400. 100 g
- Benzoyl Peroxide, damped. 25 g Oxidising, irritant
- N,N-Dimethylaniline. 25 g Toxic
Technical Note
 | Download Technical Note |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1036 | Glycol Methacrylate Resin Kit | Each | p.o.r. |
Histocryl Resin Kit
Histocryl is a hydrophilic acrylic resin of low toxicity which is easy to use and formulated specifically for light microscopy.
It permits the use of most routine staining techniques without prior removal or etching of the resin.
For those laboratories using other multi-component acrylic systems, no alteration to processing schedules is necessary.
The kit consists of:
- Histocryl. 500g Irritant
- Catalyst. 50g Oxidising, irritant
- Accelerator. 10ml Toxic
Technical Note
| Download Technical Note |
MSDS
| Download Material Safety Data Sheet – R1131A Histocryl Accelerator |
| Download Material Safety Data Sheet – R1131B Benzoyl Peroxide Catalyst |
| Download Material Safety Data Sheet – R1131H Histocryl |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1131 | Histocryl Resin Kit | Each | p.o.r. |
LR White Resin
This is a hydrophilic acrylic resin of low viscosity (8 cps) available in three grades of hardness.
The resin has wide application, being suitable for both light and electron microscopy.
With appropriate fixation, the same specimen may be used for both techniques although the soft grade is not recommended for EM use.
It may be thermally cured or cold cured using the accelerator.
It has been successfully used for immunocytochemical and immunohistochemical techniques but it is not designed to be used at very low temperatures since the components begin to separate out at about -15 C.
Its low viscosity makes it suitable for infiltrating decalcified bone and teeth.
LR White is available in either a convenient premixed and ready to use form, Catalysed LR White, or as a non-catalysed version.
The uncatalysed version of LR resin does not need to be kept in a fridge and can be kept at room temperature in a dry place. 24 hours prior to use, simply add in the catalyst (9.9g of catalyst per 500ml of LR White), mix thoroughly and leave for 24 hours to ensure the catalyst has fully dissolved into the resin.
The catalysed version of LR White should be kept at 4 C or lower (air shipment or courier for export).
The resin is an irritant and the accelerator is toxic.
Technical Note
| Download Technical Note – Using LR White for Electron Microscopy |
| Download Technical Note – Using LR White for Hard Tissue |
| Download Technical Note – Using LR White for Light Microscopy |
| Download Technical Note – Using LR White for Immunohistochemistry |
| Download Technical Note – Using LR White for Electron-Microscopic Immunocytochemistry |
MSDS
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1282 | London Resin (Soft) | 500g | p.o.r. | ||
| R1281 | London Resin (Medium) | 500g | p.o.r. | ||
| R1280 | London Resin (Hard) | 500g | p.o.r. | ||
| R1282A | LR White Resin (Soft) Uncatalysed | 500g | p.o.r. | ||
| R1280A | LR White Resin (Hard) Uncatalysed | 500g | p.o.r. | ||
| R1281A | LR White Resin (Medium) Uncatalysed | 500g | p.o.r. | ||
| R1281C | Catalyst for London Resin | 9,9g | p.o.r. | ||
| R1283 | Accelerator for London Resin | 10ml | p.o.r. |
LR Gold resin
LR Gold was originally designed primarily for embedding unfixed tissue at low temperature.
This enables enzyme histochemistry and immunocytochemistry of many fixation-sensitive and temperaturelabile enzymes and antigens to be carried out in 1 – 4 m resin sections.
It is an acrylic resin with low viscosity.
The resin may be cured at temperatures down to -25 C using a visible light curing source and benzil, a light sensitive initiator.
It is recommended that polyvinylpyrrolidone (PVP) is used to protect unfixed tissue from osmotic changes during processing.
It has also been found to have certain advantages over other resins for use with fixed tissue.
It can be used in place of LR White protocols when lower temperatures are required.
Technical Note
| Download Technical Note – Technical Note |
MSDS
| Download Material Safety Data Sheet – R1284 LR Gold Resin |
| Download Material Safety Data Sheet – R1285 Benzil |
| Download Material Safety Data Sheet – R1286 Poly(vinylpyrrolidone) |
| Download Material Safety Data Sheet – R1287 Benzoyl Peroxide (stabilised 50%) |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1284 | LR Gold Resin | 500g | p.o.r. | ||
| R1285 | Activator – Benzil | 50g | p.o.r. | ||
| R1286 | Polyvinylpyrrolidone | 100g | p.o.r. | ||
| R1287 | HAZ Benzoyl Peroxide (stabilised 50%) | 50g | p.o.r. |
Epoxy resin (Araldite CY212) Kit
Araldite – this epoxy resin is also known as Araldite M. This kit has proved to be extremely reliable and versatile for a range of tissues.
It is easy to section.
The sections are stable in the electron beam.
The kit consists of:
- Epoxy resin (Araldite CY212). 5 x 100g Irritant
- DDSA. 5 x 100g Irritant
- Dibutyl phthalate. 100g Toxic
- BDMA. 50g Corrosive
Technical Note
| Download Technical Note |
MSDS
| Download Material Safety Data Sheet – R1030B – BDMA |
| Download Material Safety Data Sheet – R1030C – Dibutyl phthalate |
| Download Material Safety Data Sheet – R1030D – DDSA |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1030 | Epoxy resin Araldite CY212 Kit | Each | p.o.r. |
Agar 100 Resin Kit
Agar 100 resin is made to the same formulation as Epon 812 but it is more closely controlled with respect to viscosity; successive batches have a consistently low viscosity.
The kit consists of:
- Agar 100 Resin. 5 x 100 g Irritant
- Hardener, DDSA, 3 x 100 g Irritant
- Hardener, MNA, 3 x 100 g Corrosive
- Accelerator, BDMA, 50 g Corrosive
Technical Note
| Download Technical Note |
[pdf-embedder url=”https://localhost/loeneninstruments/pdf/AGR1036-tech-data-june21.pdf”] [pdf-embedder url=”https://localhost/loeneninstruments/wp-content/uploads/2019/12/AGR1036-tech-data-june21.pdf”]
MSDS
| Download Material Safety Data Sheet – R1053 Dodecenylsuccinic Anhydride |
| Download Material Safety Data Sheet – R1062 BDMA Benzyldimethylamine |
| Download Material Safety Data Sheet – R1083 Methyl Nadic Anhydride |
| Download Material Safety Data Sheet – R1045 Agar 100 Resin |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1031 | Agar 100 Resin Kit | Each | p.o.r. |
Agar Low Viscosity Resin Kit
ERL 4206, the major component of Spurr resin, is no longer available.
However, this Agar low viscosity resin has all the same advantages of Spurr resin.
It gives rapid penetration, good contrast, ease of sectioning, stability under the electron beam and good staining characteristics.
It can be used for both optical and electron microscopy applications.
The hardness of the block can be adjusted by changing the ratios of the two hardeners VH1 and VH2.
None of the components has the known carcinogenicity of ERL 4206 but, as with the laboratory use of all resins, care should be taken at all stages of handling with the use of gloves and a well ventilated area.
The kit consists of:
- Resin. 5 x 100 g Irritant
- VH1 hardener. 4 x 100 g Irritant
- VH2 hardener. 2 x 100 g Corrosive
- Accelerator. 50 g Corrosive
Technical Note
| Download Technical Note |
MSDS
| Download Material Safety Data Sheet – R1373 Succininic anhydride |
| Download Material Safety Data Sheet – R1378 Hardener, MNA |
| Download Material Safety Data Sheet – R1370 Low Viscosity Resin |
| Download Material Safety Data Sheet – R1379 BDMA |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1078 | Agar Low Viscosity Resin Kit | Each | p.o.r. |
Pre-mix resin kits
These kits make approximately 500g of resin and have the advantage that the resin components are already pre-weighed.
Each kit comprises five sets of two components plus five ampoules of accelerator.
One component is supplied in a 100ml capacity bottle and the other in a 50ml bottle.
The contents of the small bottle are added to the contents of the large bottle and mixed.
An ampoule of accelerator is added and mixed thoroughly.
No additional containers are required. This makes approximately 100g of resin.
Any unused resin can be left to gel in the bottle to be discarded.
Technical Note
| Download Technical Note – R1140, R1141 & R1142 Agar 100 Premix Kit Technical Datasheet |
| Download Material Technical Note – R1143, R1144 & R1145 Araldite CY212 Premix Kit Technical Datasheet |
| Download Technical Note – R1164, R1165 & R1166 Agar Low Viscosity Premix Kit Technical Datasheet |
MSDS
AGAR 100 PREMIX KIT – HARD (AGR1140), AGAR 100 PREMIX KIT – MEDIUM (AGR1141) & AGAR 100 PREMIX KIT – SOFT (AGR1142):
| Download Material Safety Data Sheet – R1053 Dodecenylsuccinic Anhydride |
| Download Material Safety Data Sheet – R1062 BDMA Benzyldimethylamine |
| Download Material Safety Data Sheet – R1083 Methyl Nadic Anhydride |
| Download Material Safety Data Sheet – R1045 Agar 100 Resin |
ARALDITE CY212 PREMIX KIT – HARD (AGR1143), ARALDITE CY212 PREMIX KIT – MEDIUM (AGR1144) & ARALDITE CY212 PREMIX KIT – SOFT (AGR1145):
| Download Material Safety Data Sheet – Araldite Resin CY212 |
| Download Material Safety Data Sheet – R1030B – BDMA |
| Download Material Safety Data Sheet – R1030C – Dibutyl phthalate |
| Download Material Safety Data Sheet – R1030D |
AGAR LOW VISCOSITY PREMIX KIT – HARD (AGR1164), AGAR LOW VISCOSITY PREMIX KIT – MEDIUM (AGR1165) & AGAR LOW VISCOSITY PREMIX KIT – SOFT (AGR1166):
| Download Material Safety Data Sheet – R1373 Succininic anhydride |
| Download Material Safety Data Sheet – R1378 Hardener, MNA |
| Download Material Safety Data Sheet – R1370 Low Viscosity Resin |
| Download Material Safety Data Sheet – R1379 BDMA |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1140 | Agar 100 Premix Kit – Hard | Each | p.o.r. | ||
| R1141 | Agar 100 Premix Kit – Medium | Each | p.o.r. | ||
| R1142 | Agar 100 Premix Kit – Soft | Each | p.o.r. | ||
| R1143 | Araldite CY212 Premix Kit – Hard | Each | p.o.r. | ||
| R1144 | Araldite CY212 Premix Kit – Medium | Each | p.o.r. | ||
| R1145 | Araldite CY212 Premix Kit – Soft | Each | p.o.r. | ||
| R1164 | Agar Low Viscosity Premix Kit Hard | Each | p.o.r. | ||
| R1165 | Agar Low Viscosity Premix Kit Medium | Each | p.o.r. | ||
| R1166 | Agar Low Viscosity Premix Kit Soft | Each | p.o.r. |
Lowicryl resin kits for low temperature embedding
These Lowicryl embedding resins are based on a highly cross-linked acrylate and methacrylate formula developed by Carlemalm et al in Professor Kellenberger’s laboratory. They have been designed for use over a wide range of embedding conditions and have been formulated to provide low viscosity at low temperature; K4M down to -35 C and HM20 to -70 C. K4M is a water compatible polar embedding resin while HM20 is a hydrophobic non-polar resin.
Both resins may be polymerised by ultraviolet light (360nm) at low temperatures or at room temperature.
Thermal polymerisation is also possible. Dehydration, infiltration and embedding are carried out at low temperatures reducing the extraction of lipids.
These resins permit adequate retention of ultrastructure, together with improved preservation of antigenicity.
K4M kit:
- K4M resin (monomer B). 3 x 250ml
- K4M cross-linker (A). 130ml
- K4M initiator (C). 5g
HM20 kit:
- HM20 resin (monomer E). 3 x 250ml
- HM20 cross-linker (D). 130ml
- HM20 initiator (C). 5g
Lowicryl K11M and HM23 kits have similar properties to K4M and HM20 but have been developed for use at significantly lower temperatures.
They are based on acrylate and methacrylate esters with freezing points that allow applications down to approx -60 C and -80 C for K11M and HM23 respectively.
K11M is hydrophilic and HM23 hydrophobic. Both resins have been specifically designed for freeze substitution, together with low temperature embedding.
K11M has a lower viscosity than K4M.
K11M kit:
- K11M resin (monomer I). 3 x 250ml
- K11M cross-linker (H). 50ml
- K11M initiator (C). 5g
HM23 kit:
- HM23 resin (monomer G). 250ml
- HM23 cross-linker (F). 130ml
- HM23 initiator (C). 5g (for polymerisation above -50 C)
- HM23 initiator (J). 5g (for polymerisation -50 C and below
MSDS
| Download Material Safety Data Sheet – R1034 Initiator C (Lowicryl HM20) |
| Download Material Safety Data Sheet – R1034D Crosslinker D (Lowicryl HM20) |
| Download Material Safety Data Sheet – R1034E Monomer E Solution (Lowicryl HM20) |
| Download Material Safety Data Sheet – R1035B Crosslinker A (Lowicryl K4M) |
| Download Material Safety Data Sheet – R1035A Monomer B Solution (Lowicryl K4M) |
| Download Material Safety Data Sheet – R1133C Crosslinker H Lowicryl K11M |
| Download Material Safety Data Sheet – R1133M Monomer I Solution (Lowicryl K11M) |
| Download Material Safety Data Sheet – R1133R Monomer I Solution (Lowicryl K11M) |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1034 | Lowicryl HM20 Kit | Each | p.o.r. | ||
| R1134 | Lowicryl HM23 Kit | Each | p.o.r. | ||
| R1133 | Lowicryl K11M Kit | Each | p.o.r. | ||
| R1035 | Lowicryl K4M Kit | Each | p.o.r. |
Lowicryl MonoStep kits
These pre-mixed, ready-to-use embedding kits save time and minimise chemical contact.
They are based on Lowicryl K4M and HM20 formulations for low temperature embedding or freeze substitution.
MSDS
| Download Material Safety Data Sheet – R1075 Lowicryl Monostep K4M Polar |
| Download Material Safety Data Sheet – R1076 Monostep Lowicryl HM20-Non Polar Embedding Media |
| Art. | Description | Unit | Price | Quantity | |
|---|---|---|---|---|---|
| R1075 | Lowicryl K4M MonoStep Kit 225g | 225g | p.o.r. | ||
| R1076 | Lowicryl HM20 MonoStep Kit 225g | 225g | p.o.r. |