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Embedding kits

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Embedding kits

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Product
Glycol Methacrylate Resin Kit
GMA (2-Hydroxyethyl methacrylate) is useful as an embedding medium for light microscopy studies.
no. R1036

Description

Glycol Methacrylate Resin Kit

The kit consists of:

  • GMA (2-Hydroxyethyl methacrylate). 5 x 100 g Irritant
  • 2-Butoxyethanol. 100 g Harmful
  • Carbowax 400. 100 g
  • Benzoyl Peroxide, damped. 25 g Oxidising, irritant
  • N,N-Dimethylaniline. 25 g Toxic
Technical Note

Technical Note

Download Technical Note
Order

Art.Description Unit PriceQuantity
R1036

Glycol Methacrylate Resin Kit

 Each p.o.r.
Brand:

Product
Histocryl Resin Kit
Histocryl is a hydrophilic acrylic resin of low toxicity which is easy to use and formulated specifically for light microscopy.
no. R1131

Description

Histocryl Resin Kit

Histocryl is a hydrophilic acrylic resin of low toxicity which is easy to use and formulated specifically for light microscopy.
It permits the use of most routine staining techniques without prior removal or etching of the resin.

For those laboratories using other multi-component acrylic systems, no alteration to processing schedules is necessary.

The kit consists of:

  • Histocryl. 500g Irritant
  • Catalyst. 50g Oxidising, irritant
  • Accelerator. 10ml Toxic
Technical Note

Technical Note

Download Technical Note
MSDS

MSDS

Download Material Safety Data Sheet – R1131A Histocryl Accelerator
Download Material Safety Data Sheet – R1131B Benzoyl Peroxide Catalyst
Download Material Safety Data Sheet – R1131H Histocryl
Order

Art.Description Unit PriceQuantity
R1131

Histocryl Resin Kit

 Each p.o.r.
Brand:

Product
LR White
This is a hydrophilic acrylic resin of low viscosity with wide applications suitable for both light and electron microscopy.
no. R1280 to R1283

Description

LR White Resin

This is a hydrophilic acrylic resin of low viscosity (8 cps) available in three grades of hardness.

The resin has wide application, being suitable for both light and electron microscopy.
With appropriate fixation, the same specimen may be used for both techniques although the soft grade is not recommended for EM use.

It may be thermally cured or cold cured using the accelerator.
It has been successfully used for immunocytochemical and immunohistochemical techniques but it is not designed to be used at very low temperatures since the components begin to separate out at about -15 C.

Its low viscosity makes it suitable for infiltrating decalcified bone and teeth.

LR White is available in either a convenient premixed and ready to use form, Catalysed LR White, or as a non-catalysed version.
The uncatalysed version of LR resin does not need to be kept in a fridge and can be kept at room temperature in a dry place. 24 hours prior to use, simply add in the catalyst (9.9g of catalyst per 500ml of LR White), mix thoroughly and leave for 24 hours to ensure the catalyst has fully dissolved into the resin.

The catalysed version of LR White should be kept at 4 C or lower (air shipment or courier for export).

The resin is an irritant and the accelerator is toxic.

Technical Note

Technical Note

Download Technical Note – Using LR White for Electron Microscopy
Download Technical Note – Using LR White for Hard Tissue
Download Technical Note – Using LR White for Light Microscopy
Download Technical Note – Using LR White for Immunohistochemistry
Download Technical Note – Using LR White for Electron-Microscopic Immunocytochemistry
MSDS

MSDS

Download Material Safety Data Sheet – R1280, R1281, R1282 LR White Resin, Catalysed
Download Material Safety Data Sheet – AGR1280A, R1281A, R1281A LR White Resin, Uncatalysed
Download Material Safety Data Sheet – AGR1281C Catalyst for LR Resin
Download Material Safety Data Sheet – R1283 LR White Accelerator
Order

Art.Description Unit PriceQuantity
R1282

London Resin (Soft)

 500g p.o.r.
R1281

London Resin (Medium)

 500g p.o.r.
R1280

London Resin (Hard)

 500g p.o.r.
R1282A

LR White Resin (Soft) Uncatalysed

 500g p.o.r.
R1280A

LR White Resin (Hard) Uncatalysed

 500g p.o.r.
R1281A

LR White Resin (Medium) Uncatalysed

 500g p.o.r.
R1281C

Catalyst for London Resin

 9,9g p.o.r.
R1283

Accelerator for London Resin

 10ml p.o.r.
Brand:

Product
LR Gold
LR Gold was originally designed primarily for embedding unfixed tissue at low temperature.
no. R1284 to R1287

Description

LR Gold resin

LR Gold was originally designed primarily for embedding unfixed tissue at low temperature.

This enables enzyme histochemistry and immunocytochemistry of many fixation-sensitive and temperaturelabile enzymes and antigens to be carried out in 1 – 4 m resin sections.

It is an acrylic resin with low viscosity.
The resin may be cured at temperatures down to -25 C using a visible light curing source and benzil, a light sensitive initiator.

It is recommended that polyvinylpyrrolidone (PVP) is used to protect unfixed tissue from osmotic changes during processing.
It has also been found to have certain advantages over other resins for use with fixed tissue.
It can be used in place of LR White protocols when lower temperatures are required.

Technical Note

Technical Note

Download Technical Note – Technical Note
MSDS

MSDS

Download Material Safety Data Sheet – R1284 LR Gold Resin
Download Material Safety Data Sheet – R1285 Benzil
Download Material Safety Data Sheet – R1286 Poly(vinylpyrrolidone)
Download Material Safety Data Sheet – R1287 Benzoyl Peroxide (stabilised 50%)
Order

Art.Description Unit PriceQuantity
R1284

LR Gold Resin

 500g p.o.r.
R1285

Activator – Benzil

 50g p.o.r.
R1286

Polyvinylpyrrolidone

 100g p.o.r.
R1287

HAZ Benzoyl Peroxide (stabilised 50%)

 50g p.o.r.
Brand:

Product
Epoxy resin (Araldite CY212) Kit
This epoxy resin is also known as Araldite M.
no. R1030

Description

Epoxy resin (Araldite CY212) Kit

Araldite – this epoxy resin is also known as Araldite M. This kit has proved to be extremely reliable and versatile for a range of tissues.

It is easy to section.
The sections are stable in the electron beam.

The kit consists of:

  • Epoxy resin (Araldite CY212). 5 x 100g Irritant
  • DDSA. 5 x 100g Irritant
  • Dibutyl phthalate. 100g Toxic
  • BDMA. 50g Corrosive
Technical Note

Technical Note

Download Technical Note
MSDS

MSDS

Download Material Safety Data Sheet – R1030B – BDMA
Download Material Safety Data Sheet – R1030C – Dibutyl phthalate
Download Material Safety Data Sheet – R1030D – DDSA
Order

Art.Description Unit PriceQuantity
R1030

Epoxy resin Araldite CY212 Kit

 Each p.o.r.
Brand:

Product
Agar 100 Resin Kit
Agar 100 resin is made to the same formulation as Epon 812 but it is more closely controlled with respect to viscosity
no. R1031

Description

Agar 100 Resin Kit

Agar 100 resin is made to the same formulation as Epon 812 but it is more closely controlled with respect to viscosity; successive batches have a consistently low viscosity.

The kit consists of:

  • Agar 100 Resin. 5 x 100 g Irritant
  • Hardener, DDSA, 3 x 100 g Irritant
  • Hardener, MNA, 3 x 100 g Corrosive
  • Accelerator, BDMA, 50 g Corrosive
Technical Note

Technical Note

Download Technical Note

[pdf-embedder url=”https://localhost/loeneninstruments/pdf/AGR1036-tech-data-june21.pdf”] [pdf-embedder url=”https://localhost/loeneninstruments/wp-content/uploads/2019/12/AGR1036-tech-data-june21.pdf”]

MSDS

MSDS

Download Material Safety Data Sheet – R1053 Dodecenylsuccinic Anhydride
Download Material Safety Data Sheet – R1062 BDMA Benzyldimethylamine
Download Material Safety Data Sheet – R1083 Methyl Nadic Anhydride
Download Material Safety Data Sheet – R1045 Agar 100 Resin
Order

Art.Description Unit PriceQuantity
R1031

Agar 100 Resin Kit

 Each p.o.r.
Brand:

Product
Agar Low Viscosity Resin Kit
This Agar low viscosity resin has all the same advantages of Spurr resin.
no. R1078

Description

Agar Low Viscosity Resin Kit

ERL 4206, the major component of Spurr resin, is no longer available.
However, this Agar low viscosity resin has all the same advantages of Spurr resin.

It gives rapid penetration, good contrast, ease of sectioning, stability under the electron beam and good staining characteristics.
It can be used for both optical and electron microscopy applications.

The hardness of the block can be adjusted by changing the ratios of the two hardeners VH1 and VH2.
None of the components has the known carcinogenicity of ERL 4206 but, as with the laboratory use of all resins, care should be taken at all stages of handling with the use of gloves and a well ventilated area.

The kit consists of:

  • Resin. 5 x 100 g Irritant
  • VH1 hardener. 4 x 100 g Irritant
  • VH2 hardener. 2 x 100 g Corrosive
  • Accelerator. 50 g Corrosive
Technical Note

Technical Note

Download Technical Note
MSDS

MSDS

Download Material Safety Data Sheet – R1373 Succininic anhydride
Download Material Safety Data Sheet – R1378 Hardener, MNA
Download Material Safety Data Sheet – R1370 Low Viscosity Resin
Download Material Safety Data Sheet – R1379 BDMA
Order

Art.Description Unit PriceQuantity
R1078

Agar Low Viscosity Resin Kit

 Each p.o.r.
Brand:

Product
Premix Resin Kits
These kits make approximately 500 g of resin and have the advantage that the resin components are already pre-weighed.
no. R1140 to R1166

Description

Pre-mix resin kits

These kits make approximately 500g of resin and have the advantage that the resin components are already pre-weighed.
Each kit comprises five sets of two components plus five ampoules of accelerator.

One component is supplied in a 100ml capacity bottle and the other in a 50ml bottle.

The contents of the small bottle are added to the contents of the large bottle and mixed.
An ampoule of accelerator is added and mixed thoroughly.

No additional containers are required. This makes approximately 100g of resin.
Any unused resin can be left to gel in the bottle to be discarded.

Technical Note

Technical Note

Download Technical Note – R1140, R1141 & R1142 Agar 100 Premix Kit Technical Datasheet
Download Material Technical Note – R1143, R1144 & R1145 Araldite CY212 Premix Kit Technical Datasheet
Download Technical Note – R1164, R1165 & R1166 Agar Low Viscosity Premix Kit Technical Datasheet
MSDS

MSDS

AGAR 100 PREMIX KIT – HARD (AGR1140), AGAR 100 PREMIX KIT – MEDIUM (AGR1141) & AGAR 100 PREMIX KIT – SOFT (AGR1142):

Download Material Safety Data Sheet – R1053 Dodecenylsuccinic Anhydride
Download Material Safety Data Sheet – R1062 BDMA Benzyldimethylamine
Download Material Safety Data Sheet – R1083 Methyl Nadic Anhydride
Download Material Safety Data Sheet – R1045 Agar 100 Resin

ARALDITE CY212 PREMIX KIT – HARD (AGR1143), ARALDITE CY212 PREMIX KIT – MEDIUM (AGR1144) & ARALDITE CY212 PREMIX KIT – SOFT (AGR1145):

Download Material Safety Data Sheet – Araldite Resin CY212
Download Material Safety Data Sheet – R1030B – BDMA
Download Material Safety Data Sheet – R1030C – Dibutyl phthalate
Download Material Safety Data Sheet – R1030D

AGAR LOW VISCOSITY PREMIX KIT – HARD (AGR1164), AGAR LOW VISCOSITY PREMIX KIT – MEDIUM (AGR1165) & AGAR LOW VISCOSITY PREMIX KIT – SOFT (AGR1166):

Download Material Safety Data Sheet – R1373 Succininic anhydride
Download Material Safety Data Sheet – R1378 Hardener, MNA
Download Material Safety Data Sheet – R1370 Low Viscosity Resin
Download Material Safety Data Sheet – R1379 BDMA
Order

Art.Description Unit PriceQuantity
R1140

Agar 100 Premix Kit – Hard

 Each p.o.r.
R1141

Agar 100 Premix Kit – Medium

 Each p.o.r.
R1142

Agar 100 Premix Kit – Soft

 Each p.o.r.
R1143

Araldite CY212 Premix Kit – Hard

 Each p.o.r.
R1144

Araldite CY212 Premix Kit – Medium

 Each p.o.r.
R1145

Araldite CY212 Premix Kit – Soft

 Each p.o.r.
R1164

Agar Low Viscosity Premix Kit Hard

 Each p.o.r.
R1165

Agar Low Viscosity Premix Kit Medium

 Each p.o.r.
R1166

Agar Low Viscosity Premix Kit Soft

 Each p.o.r.
Brand:

Product
Lowicryl Kits
These embedding resins are based on a highly cross-linked acrylate and methacrylate formula developed by Carlemalm et al in Professor Kellenberger's laboratory.
no. R1034 to R1133

Description

Lowicryl resin kits for low temperature embedding

These Lowicryl embedding resins are based on a highly cross-linked acrylate and methacrylate formula developed by Carlemalm et al in Professor Kellenberger’s laboratory. They have been designed for use over a wide range of embedding conditions and have been formulated to provide low viscosity at low temperature; K4M down to -35 C and HM20 to -70 C. K4M is a water compatible polar embedding resin while HM20 is a hydrophobic non-polar resin.

Both resins may be polymerised by ultraviolet light (360nm) at low temperatures or at room temperature.
Thermal polymerisation is also possible. Dehydration, infiltration and embedding are carried out at low temperatures reducing the extraction of lipids.
These resins permit adequate retention of ultrastructure, together with improved preservation of antigenicity.

K4M kit:

  • K4M resin (monomer B). 3 x 250ml
  • K4M cross-linker (A). 130ml
  • K4M initiator (C). 5g

 

HM20 kit:

  • HM20 resin (monomer E). 3 x 250ml
  • HM20 cross-linker (D). 130ml
  • HM20 initiator (C). 5g

 

Lowicryl K11M and HM23 kits have similar properties to K4M and HM20 but have been developed for use at significantly lower temperatures.
They are based on acrylate and methacrylate esters with freezing points that allow applications down to approx -60 C and -80 C for K11M and HM23 respectively.

K11M is hydrophilic and HM23 hydrophobic. Both resins have been specifically designed for freeze substitution, together with low temperature embedding.
K11M has a lower viscosity than K4M.

K11M kit:

  • K11M resin (monomer I). 3 x 250ml
  • K11M cross-linker (H). 50ml
  • K11M initiator (C). 5g

 

HM23 kit:

  • HM23 resin (monomer G). 250ml
  • HM23 cross-linker (F). 130ml
  • HM23 initiator (C). 5g (for polymerisation above -50 C)
  • HM23 initiator (J). 5g (for polymerisation -50 C and below
MSDS

MSDS

Download Material Safety Data Sheet – R1034 Initiator C (Lowicryl HM20)
Download Material Safety Data Sheet – R1034D Crosslinker D (Lowicryl HM20)
Download Material Safety Data Sheet – R1034E Monomer E Solution (Lowicryl HM20)
Download Material Safety Data Sheet – R1035B Crosslinker A (Lowicryl K4M)
Download Material Safety Data Sheet – R1035A Monomer B Solution (Lowicryl K4M)
Download Material Safety Data Sheet – R1133C Crosslinker H Lowicryl K11M
Download Material Safety Data Sheet – R1133M Monomer I Solution (Lowicryl K11M)
Download Material Safety Data Sheet – R1133R Monomer I Solution (Lowicryl K11M)
Order

Art.Description Unit PriceQuantity
R1034

Lowicryl HM20 Kit

 Each p.o.r.
R1134

Lowicryl HM23 Kit

 Each p.o.r.
R1133

Lowicryl K11M Kit

 Each p.o.r.
R1035

Lowicryl K4M Kit

 Each p.o.r.
Brand:

Product
Lowicryl Monostep Kits
These pre-mixed, ready-to-use embedding kits save time and minimise chemical contact.
no. R1075 and R1076

Description

Lowicryl MonoStep kits

These pre-mixed, ready-to-use embedding kits save time and minimise chemical contact.
They are based on Lowicryl K4M and HM20 formulations for low temperature embedding or freeze substitution.

MSDS

MSDS

Download Material Safety Data Sheet – R1075 Lowicryl Monostep K4M Polar
Download Material Safety Data Sheet – R1076 Monostep Lowicryl HM20-Non Polar Embedding Media
Order

Art.Description Unit PriceQuantity
R1075

Lowicryl K4M MonoStep Kit 225g

 225g p.o.r.
R1076

Lowicryl HM20 MonoStep Kit 225g

 225g p.o.r.
Brand: