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Home / Accessories for Microscopy / Chemicals / Embedding / Embedding Components

Embedding Components

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Embedding Components

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Product
Description
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Product

Polyvinyl Alcohol
no. R1350

Description

Polyvinyl Alcohol molecular weight 30,000 – 70,000, 250g

It is used as an alternative to methyl cellulose in cryo-ultramicrotomy.

Ref: Tokuyasu, K.T. ‘The use of PVA pyrrolidone and PVA for cryo-ultramicrotomy’. Histochem J. 21, 163-171. 1989.

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Art.Description Unit PriceQuantity
R1350

Polyvinyl Alcohol Mol. Wt. 30,000 70,000

250g € 59,90
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Brand: Agar

Product
Description
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Product

OCT Compound
Embedding medium for frozen tissue specimens.
no. R1180

Description

OCT Compound 118ml

Embedding medium for frozen tissue specimens.
OCT Compound is a water-soluble blend of glycols and resins that provides a convenient specimen matrix for cryostat sectioning at temperatures of -10?C and below.
OCT leaves no residue on slides during staining procedure, eliminating undesirable background staining.

Dissected tissue, mounted in OCT Compound can be frozen between -20 and -80?C.
Developed and optimized for chromogenic IHC experiments, OCT Compound will not dull microtome knives.

OCT Compound contains:

  • Polyvinyl alcohol
  • Polyethylene glycol
  • Nonreactive ingredients

 

Supplied in 118ml (4oz) squeeze bottles.

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Art.Description Unit PriceQuantity
R1180

OCT Compound

118ml € 28,40
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Brand: Agar

Product
Description
Technical Note
MSDS
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Product

Epoxy Resins
Epoxy resins for the preparation and sectioning of specimens for TEM.
no. R1040 to R1372

Description

Epoxy resins and methacrylates are often harmful. Resin blocks are harmless after curing but, if cured blocks are sawn, make sure that the dust is not inhaled.

Agar 100 – replaces Epon 812 resin, now unobtainable. The formulation of Agar 100 resin is the same as Epon, but is less viscous than many samples of Epon 812 and is more closely controlled in its manufacture. Epoxide equivalent between 148 and 150.

ERL 4221 has replaced ERL4206. It has a higher viscosity (180cps) than the previously used ERL 4206 (60cps). Ref: Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinylcyclohexene dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, Vol 14, No 4, July 2006.

Technical Note

Technical Note

The original formulation, ERL 4206 (VCD) – DER® 736 embedding medium devised by Spurr (1969) combined very low viscosity components for rapid specimen penetration.

This embedding medium has been especially suitable for a variety of mineral and biological specimens. Since ERL 4206 is no longer available, ERL 4221 has been substituted as a replacement*.

It will work well for most users but it does have somewhat higher viscosity (180cp vs. 60cp) than the ERL 4206, which can impact some protocols employing vacuum embedding.

*Reference: Ellis, E. Ann Microscopy Today, July, 33 (2006)

A block of MEDIUM hardness can be obtained by using the following formula based on a batch of 10 grams without the flexibilizer, DER 736:

Part no. Description Hardness Rapid
Medium Harder Softer
AGR1047R ERL 4221 4.10 g
AGR1072/73/74 DER 736 Diglycidylether of Polypropyleneglycol 1.43 g 0.95 g 1.90 g
AGR1054/55/56 NSA (Nonenyl Succinic Anhydride) redistilled 5.90 g
AGR1066/67 S-1 (Dimethylaminoethanol) 0.10 g 0.20 g

 

A harder block can be produced by decreasing the DER® 736 component to 0.95g in the above formula; a softer block by increasing the DER 736 to 1.90g.

ALL STEPS MUST BE PERFORMED UNDER A HOOD AND GLOVES MUST BE WORN FOR PROTECTION.

Preparation:

Care should be taken to weigh the ingredients accurately into a beaker. The quantities given in the first column are for firm blocks.
For harder blocks, decrease the DER 736 to 0.95g; for softer blocks, increase the DER 736 to 1.90g.

For rapid cure, increase the DMAE. It is recommended to add the catalyst (dimethylaminoethanol) last, after having carefully mixed the other components.
If bubbles are a problem, they may be eliminated by placing the beaker into a desiccator under a gentle vacuum.

MSDS

MSDS

Download Material Safety Data Sheet – AGR1040, AGR1041 & AGR1042 Araldite Resin CY212
Download Material Safety Data Sheet – AGR1043, AGR1044 & AGR1045 Agar 100 Resin
Download Material Safety Data Sheet – AGR1370, AGR1371 & AGR1372 Low Viscosity Resin
Download Material Safety Data Sheet – AGR1047R ERL-4221
Order

Art.Description Unit PriceQuantity
R1040

Araldite CY212 Resin

500g € 48,40
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R1041

Araldite CY212 Resin

250g € 29,30
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R1042

Araldite CY212 Resin

100g € 12,80
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R1043

Agar 100 Resin

500g € 120,60
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R1044

Agar 100 Resin

250g € 68,30
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R1045

Agar 100 Resin

100g € 32,20
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R1047R

ERL 4221

225ml € 63,10
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R1090

Araldite RD-2 Resin

500g € 73,60
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R1091

Araldite RD-2 Resin

250g € 38,60
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R1092

Araldite RD-2 Resin

100g € 16,30
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R1370

Agar Low Viscosity Resin

500g € 113,40
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R1371

Agar Low Viscosity Resin

250g € 70,40
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R1372

Agar Low Viscosity Resin

100g € 33,90
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Quetol 651
This is an epoxy resin with a viscosity of 15 cps at 25 C and an epoxy equivalent of 115.
no. R1068

Description

Quetol 651 500g

This is an epoxy resin with a viscosity of 15 cps at 25 C and an epoxy equivalent of 115. It is miscible with water, alcohol and acetone.

When used with NSA and DMP-30, it forms a low viscosity mix which penetrates tissue easily.

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Art.Description Unit PriceQuantity
R1068

Quetol 651

500g € 219,70
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Description
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Hardeners for Epoxy Resins
Hardeners for epoxy resins
no. R1051 to R1378

Description

Hardeners for epoxy resins

DDSA, NSA + VH1 are irritants.
MNA + VH2 are corrosive.

MSDS

MSDS

Download Material Safety Data Sheet – AGR1051, AGR1052 & AGR1053 Dodecenylsuccinic Anhydridee
Download Material Safety Data Sheet – AGR1054, AGR1055 & AGR1056 Nonenyl Succinic Anhydride
Download Material Safety Data Sheet – AGR1081, AGR1082 & AGR1083 Methyl Nadic Anhydride
Download Material Safety Data Sheet – AGR1373, AGR1374 & AGR1375 Low Viscosity Hardener
Download Material Safety Data Sheet – AGR1376 Low Viscosity Hardener VH2
Download Material Safety Data Sheet – AGR1377 Low Viscosity Hardener VH2
Download Material Safety Data Sheet – AGR1378 Low Viscosity Hardener VH2
Order

Art.Description Unit PriceQuantity
R1051

DDSA (Dodecenyl Succinic Anhydride) EM grade redistilled

500g € 74,90
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R1052

DDSA (Dodecenyl Succinic Anhydride) EM grade redistilled

250g € 39,40
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R1053

DDSA (Dodecenyl Succinic Anhydride) EM grade redistilled

100g € 16,50
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R1054

NSA (Nonenyl Succinic Anhydride) redistilled

500g € 88,30
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R1055

NSA (Nonenyl Succinic Anhydride) redistilled

250g € 43,90
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R1056

NSA (Nonenyl Succinic Anhydride) redistilled

100g € 20,40
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R1081

MNA Methyl Nadic Anhydride redistilled

500g € 46,90
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R1082

MNA Methyl Nadic Anhydride redistilled

250g € 24,40
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R1083

MNA Methyl Nadic Anhydride redistilled

100g € 11,80
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R1373

Low Viscosity Hardener VH1

500g € 84,60
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R1374

Low Viscosity Hardener VH1

250g € 49,10
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R1375

Low Viscosity Hardener VH1

100g € 20,40
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R1376

Low Viscosity Hardener VH2

500g € 49,10
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R1377

Low Viscosity Hardener VH2

250g € 25,50
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R1378

Low Viscosity Hardener VH2

100g € 12,30
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Brand: Agar

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Accelerators for Epoxy Resins
BDMA is recommended in preference to DMP-30 since it is less viscous and has a longer shelf life.
no. R1060 to R1381

Description

Accelerators for epoxy resins

BDMA is recommended in preference to DMP-30 since it is less viscous and has a longer shelf life.

Accelerators should be stored dry in a desiccator, but need not be in a refrigerator.
All these materials have a shorter shelf life than the resins and should be discarded after six months.

DMP-30 is harmful
BDMA, S-1 + accelerator (LV) are corrosive.

MSDS

MSDS

Download Material Safety Data Sheet – AGR1060, AGR1061 & AGR1062 BDMA Benzyldimethylamine
Download Material Safety Data Sheet – AGR1063 DMP-30
Download Material Safety Data Sheet – AGR1064 DMP-30
Download Material Safety Data Sheet – AGR1065 DMP-30
Download Material Safety Data Sheet – AGR1065 DMP-30 MSDS
Download Material Safety Data Sheet – AGR1066 & AGR1067 S-1 2-Dimethylaminoethanol
Download Material Safety Data Sheet – AGR1379, AGR1380 & AGR1381 LV accelerator
Order

Art.Description Unit PriceQuantity
R1060

BDMA Benzyl Dimethylamine

500g € 154,40
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R1061

BDMA Benzyl Dimethylamine

250g € 43,80
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R1062

BDMA Benzyl Dimethylamine

100g € 18,10
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R1063

DMP-30 Tri-Dimethylaminomethyl Phenol

500g € 35,10
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R1064

DMP-30 Tri-Dimethylaminomethyl Phenol

250g € 19,60
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R1065

DMP-30 Tri-Dimethylaminomethyl Phenol

100g € 10,10
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R1066

S-1 Dimethylaminoethanol

500g € 161,20
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R1067

S-1 Dimethylaminoethanol

100g € 42,00
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R1379

Low Viscosity Accelerator

100ml € 14,60
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R1380

Low Viscosity Accelerator

50ml € 10,00
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R1381

Low Viscosity Accelerator

5 x 2,5ml € 21,20
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Brand: Agar

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Description
MSDS
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Plasticisers
Dibutyl Phthalate
no. R1070 and R1071

Description

Dibutyl Phthalate Plasticisers

MSDS

MSDS

Download Material Safety Data Sheet

 

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Art.Description Unit PriceQuantity
R1070

Dibutyl Phthalate

500g € 42,00
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R1071

Dibutyl Phthalate

100g € 10,60
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Description
MSDS
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Additives
Additives - Epoxy. DER 736 is an irritant.
no. R1072 to R1089

Description

DER 736 is an irritant.

MSDS

MSDS

Download Material Safety Data Sheet
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Art.Description Unit PriceQuantity
R1072

DER 736 Diglycidylether of Polypropyleneglycol

500g € 59,80
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R1073

DER 736 Diglycidylether of Polypropyleneglycol

250g € 31,00
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R1074

DER 736 Diglycidylether of Polypropyleneglycol

100g € 13,60
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R1088

N N-Dimethyl Aniline

100g € 23,50
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R1089

2-Butoxyethanol

500g € 40,50
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Brand: Agar

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Methyl Methacrylates
Methyl methacrylate + n-Butyl meth are flammable.
no. R1049 to R1086

Description

Methacrylates

Methyl methacrylate + n-Butyl meth are flammable.
All methacrylates are irritants.

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Art.Description Unit PriceQuantity
R1049

Methyl Methacrylate

500g € 158,80
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R1050

N-Butyl Methacrylate

500g € 84,50
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R1084

GMA (2-Hydroxyethyl Methacrylate)

500g € 61,60
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R1085

GMA (2-Hydroxyethyl Methacrylate)

250g € 33,30
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R1086

GMA (2-Hydroxyethyl Methacrylate)

100g € 14,10
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Polymerisation catalyst for methacrylates
Polymerisation catalyst for methacrylates
no. R1059 and R1058

Description

Damped with 25% water.

MSDS

MSDS

Download Material Safety Data Sheet – Technical Note
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Art.Description Unit PriceQuantity
AGR1059

Benzoyl Peroxide

25g € 27,60
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AGR1058

Benzoyl Peroxide

100g € 93,60
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Description
Technical Note
MSDS
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Resin Solvents
A solvent for unpolymerised or partially polymerised resins and their components.
no. R1346 and R1347

Description

Resin solvents

A solvent for unpolymerised or partially polymerised resins and their components.

Clean glassware etc. with resin solvent and wash with water.
The solvent is water miscible.
An active solvent is also available for polymerised resins.

Technical Note

Technical Note

Download Technical Note
MSDS

MSDS

Download Material Safety Data Sheet – AGR1346 Solvent for Polymerised Resin
Download Material Safety Data Sheet – AGR1347 Solvent for Unpolymerised Resin
Order

Art.Description Unit PriceQuantity
R1347

Solvent for Unpolymerised Resin

500ml € 40,70
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R1346

Solvent for Polymerised Resin

50ml € 37,20
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Chemicals for Support Films
A variety of film-forming solutions, including Formvar, Pioloform and Butvar, to prepare support films and replicas of smooth surfaces.
no. R1201 to R1277

Description

A variety of film-forming solutions are available to prepare support films and replicas of smooth surfaces.

Formvar (polyvinyl formal) is normally used as a 0.25% solution in chloroform.
The thickness and stability of the film can be altered by adjusting the concentration between 0.25% and 0.5%.

Pioloform is polyvinyl butyral dissolved in chloroform (0.3 – 1.5%).
The solution should be stored in a refrigerator in an amber glass bottle, and brought up to room temperature before use.

Butvar B98 is a polyvinyl butyral resin containing 20% polyvinyl alcohol, and is normally used as a 0.25% solution in chloroform.
It is an alternative support film to Formvar as reported by: Handley and Olsen, Ultramicroscopy 4, p479 – 480, 1979.

Films made from Butvar are hydrophilic, and are therefore suitable for negative staining methods, having good mechanical stability, high electron transparency and minimum intrinsic structure.

Butvar B98 can also be applied to grids as an adhesive.

MSDS

MSDS

Download Material Safety Data Sheet – AGR1201 & AGR1202 Formvar
Download Material Safety Data Sheet – AGR1275 Pioloform
Download Material Safety Data Sheet – AGR1276 Butvar
Download Material Safety Data Sheet – AGR1277 Silicon Monoxide
Order

Art.Description Unit PriceQuantity
R1201

Formvar

100g € 34,50
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R1202

Formvar

25g € 9,00
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R1275

Pioloform Powder

10g € 19,30
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R1276

Butvar B98 Powder

25g € 20,30
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R1277

Silicon Monoxide

10g € 74,50
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Hexamethyldisilazane HMDS
HMDS, [(CH3)3Si]2NH, can be used in place of critical point drying for the preparation of soft tissue for SEM examination, for example, of delicate insect tissues.
no. R1228

Description

Hexamethyldisilazane (HMDS) 100ml

HMDS, [(CH3)3Si]2NH, can be used in place of critical point drying for the preparation of soft tissue for SEM examination, for example, of delicate insect tissues.

It is faster, preserves surface detail, reduces thermal and pressure stresses, and may also reduce the extraction of cellular components compared with CPD. HMDS can be used to dry specimens such as bacteria on polycarbonate filters. 100ml

Bray, D.F. et al. ‘A comparison of HMDS, Peldri II, and critical drying methods for SEM of biological specimens’. Micros. Res. And Technique. 26, 489 – 495. 1993.

MSDS

MSDS

Download Material Safety Data Sheet
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Art.Description Unit PriceQuantity
R1228

Hexamethyldisilazane HMDS

100ml € 73,30
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Indicating Silica Gel
Indicating silica gel. 1kg.
no. R1278A

Specifications

Specifications

CAS Number 7631-86-9
pH 1.5 to 5
Molecular Formila O2Si
Packaging Plastic powder jar
SMILES O=[Si]=O
Molecular Weight (g/mol) 60.083
ChEBI CHEBI:30563
Physical Form Solid
Colour Brown-Orange
Quantity 1kg
MSDS

MSDS

Download Material Safety Data Sheet – Technical Note
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Art.Description Unit PriceQuantity
R1278A

Indicating Silica Gel

1kg € 148,90
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Paraffin Wax
This clean pelleted wax, suitable for all routine processing and embedding, has a melting point of 56 - 58 C.
no. L4132 and L4133

Description

Paraffin wax

This clean pelleted wax, suitable for all routine processing and embedding, has a melting point of 56 – 58 C.

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Art.Description Unit PriceQuantity
L4132

Pelleted Paraffin Wax

1kg € 36,30
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L4133

Pelleted Paraffin Wax

10kg € 275,30
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Brand: Agar

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Paraplast Plus
This double filtered paraffin embedding medium contains plastic polymers of regulated molecular weights and a small percentage (0.8 %) of dimethyl sulphoxide (DMSO)
no. AGL4382

Description

Paraplast Plus 1kg

Paraplast Plus is a double filtered paraffin embedding medium contains plastic polymers of regulated molecular weights and a small percentage (0.8 %) of dimethyl sulphoxide (DMSO) to penetrate tissues three times faster than standard media.

It melts rapidly at 56 °C, and allows short ribbons of sections cut to 1 m, and excellent ribbon continuity with 2 m thicknesses.

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Art.Description Unit PriceQuantity
AGL4382

Paraplast Plus

1kg € 42,10
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Polyester Wax
Melting point 37 C.
no. L4184

Description

Polyester Wax 500g

Melting point 37 °C.

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Art.Description Unit PriceQuantity
L4184

Polyester Wax

500g € 252,60
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Contact

Van Loenen Instruments
Penningweg 69 E
1507 DE Zaandam

Tel :+31 75 614 90 40
E-mail  :info@loeneninstruments.com

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KvK nr. :28038099

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